Fig. 3
From: Establishment of cell lines with porcine spermatogonial stem cell properties
Characterization of immortalized porcine SSCs. a RT-PCR assay showing the transcripts of UCHL1, PLZF, THY1, VASA, DAZL, CXCR4, SV40 and PCNA in the immortalized SSCs from passage 4 (P4) to passage 12 (P12). The RNAs from 7-day-old porcine testes are used as controls, and GAPDH serves as a loading control. -RT: the negative control omitting reverse transcription but with PCR reaction. b Western blot assay showing the protein expression of UCHL1, THY1, SV40, PCNA, PLZF, VASA, DAZL and CXCR4 in the immortalized SSCs at different passages. The protein extracts from 7-day-old porcine testes are used as controls, and β-actin serves as a loading control. c Staining of Ttag-transduced SSCs (passage 20) on cytospin slides for UCHL1, PLZF, VASA, DAZL, CXCR4, SV40 and PCNA. NC: the negative control using the isotype IgG in place of the primary antibody. Bar = 50 μm