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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Establishment of cell lines with porcine spermatogonial stem cell properties

Fig. 2

Immortalized porcine SSCs undergo gradual morphological transformation during long-term culture. a Representative images of the sorted Ttag-transduced SSCs on day 2, 5, 10, 20, 50 and day 100 after seeding. Asterisks indicate the cells that were experiencing morphological transformation. Bar = 100 μm. b Cell doubling time of SSCs transduced with Ttag or Puro. Data are presented as the mean ± SEM of three independent experiments. **: P < 0.01. c Staining of Ttag-transduced SSCs (passage 30) for ɑ-tubulin and phalloidin. Bar = 50 μm

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