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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Establishment of cell lines with porcine spermatogonial stem cell properties

Fig. 1

Immortalization and enrichment of porcine SSCs. a The single-cell suspension obtained after the two-enzymatic dissociation. Bar = 100 μm. b The cell fraction after differential plating. Arrows point to the putative germ cells. Bar = 100 μm. c Maps of the vector pLOX-Ttag-iresTK (hereafter referred to as Ttag) and Ef1a-Large T-Ires-Puro (hereafter referred to as Puro). d Left: living staining of Ttag- and Puro-transduced cells for PLD6. Bar = 100 μm; Right: enrichment of PLD6+ cells by FACS. e Images of Ttag- and Puro-transduced cells after sorting. Bar = 100 μm. f Staining of Ttag-transduced PLD6+ cells for lectin DBA, UCHL1, PLZF and VASA. NC: the negative control using the isotype IgG in place of the primary antibody. Bar = 50 μm. g Percentages of sorted cells positive for porcine SSC and germ cell markers. Data are presented as the mean ± SEM of three experiments (technical triplicates)

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