Dietary encapsulated essential oils and organic acids mixture improves gut health in broiler chickens challenged with necrotic enteritis

Table 8 Effects of dietary supplementation with BLJ on gene expressions of proinflammatory cytokines, chemokines and TLR signaling pathway-related genes in the jejunums of broiler chickens challenged with NE (at 7 d after NE infection)

Items	NE¹	TLR-2	TLR-4	MyD88	TRAF-6	NF-kB	IFN-γ	TNFSF15	IL-1β	IL-8	IL-10	Tollip	PI3K	A20	SOCS-1	SOCS-6
BLJ, mg/kg
0	–	1.01^ab	1.66	1.02	1.02^a	1.06	1.01	1.00^bc	1.05	1.38	1.07	1.02^ab	1.04	1.10	1.06	1.02^ab
0	+	1.26^ab	0.88	1.01	0.96^ac	0.90	3.89	1.08^b	1.14	0.63	1.37	0.84^b	0.73	0.93	1.23	0.92^b
500	–	1.45^a	0.59	1.11	1.13^a	1.16	1.13	1.32^a	1.02	0.75	0.81	1.16^a	1.26	1.35	1.07	1.45^a
500	+	0.91^b	0.46	0.87	0.36^b	0.39	3.13	0.79^c	0.62	1.02	1.24	0.57^c	0.73	1.70	1.19	0.67^b
SEM²		0.08	0.13	0.04	0.08	0.10	0.34	0.05	0.08	0.14	0.11	0.06	0.07	0.14	0.11	0.09
Main effect
0		1.14	1.27^a	1.02	0.99	0.98	2.45	1.04	1.10	1.01	1.22	0.93	0.88	1.01	1.15	0.97
500		1.18	0.52^b	0.99	0.74	0.78	2.13	1.06	0.82	0.89	1.02	0.86	1.00	1.53	1.13	1.06
Non-challenged		1.23	1.13^a	1.07	1.07	1.11^a	1.07^b	1.16	1.04	1.07	0.94	1.09	1.15^a	1.22	1.07	1.23
Challenged		1.09	0.67^b	0.94	0.66	0.64^b	3.51^a	0.93	0.88	0.83	1.30	0.70	0.73^b	1.32	1.21	0.80
P-value³
BLJ		0.780	< 0.01	0.788	0.018	0.219	0.500	0.843	0.085	0.663	0.360	0.323	0.324	0.078	0.957	0.561
Challenged		0.339	0.017	0.118	< 0.01	< 0.01	< 0.01	< 0.01	0.316	0.381	0.107	< 0.01	< 0.01	0.737	0.525	0.013
Challenged×BLJ		0.013	0.081	0.162	< 0.01	0.068	0.358	< 0.01	0.125	0.073	0.775	< 0.01	0.319	0.361	0.914	0.044

^{a, b, c} Means within the same column with different superscripts differ significantly (P < 0.05)
¹NE Co-challenged with Eimeria spp. and Clostridium perfringens; −, without NE challenge; +, with NE challenge
²SEM standard error of the mean
³P-values represent the main effect of the diet, the main effect of NE challenged, and the interaction between the dietary treatments and NE challenged

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