Table 3 Nucleotide sequences of primers (tight junction proteins and growth factors) for quantitative real-time PCR1 assay
Name | Primer sequence (5´→3´) | GenBank accession |
|---|---|---|
Tight junctions | ||
 Claudin-1 | F: AAGTGCATGGAGGATGACCA | NM_001013611.2 |
R: GCCACTCTGTTGCCATACCA | ||
 Occludin | F:TCATCGCCTCCATCGTCTAC | NM_205128.1 |
R:TCTTACTGCGCGTCTTCTGG | ||
ZO-1 | F: TATGAAGATCGTGCGCCTCC | XM_015278981.1 |
R: GAGGTCTGCCATCGTAGCTC | ||
 Mucin-2 | F: AGCGAGATGTTGGCGATGAT | NM_001318434.1 |
R: AAGTTGCCACACAGACCACA | ||
Growth factors | ||
TGF-β3 | F:TGCGGCCAGATGAGCAT | NM_205454.1 |
R:TGCACATTCCTGCCACTGA | ||
IGF-2 | F: TGGCTCTGCTGGAAACCTAC | NM_001030342.2 |
R: ACTTGGCATGAGATGGCTTC | ||
EGFR | F: ACCAGCCTGCAGAGAATGTA | NM_205497 |
R: CACCATGTTAAGCGCAATGA | ||
GLP-2 | F:AAGCTTCCCAGTCTGAACCA | NM_205260.4 |
R:ATCCTGAGCTCGTCTGCTGT | ||
House-keeping genes | ||
 β-actin | F: GAGAAATTGTGCGTGACATCA | NM 205518 |
R: CCTGAACCTCTCATTGCCA | ||