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Table 3 Nucleotide sequences of primers (tight junction proteins and growth factors) for quantitative real-time PCR1 assay

From: Dietary encapsulated essential oils and organic acids mixture improves gut health in broiler chickens challenged with necrotic enteritis

Name

Primer sequence (5´→3´)

GenBank accession

Tight junctions

 Claudin-1

F: AAGTGCATGGAGGATGACCA

NM_001013611.2

R: GCCACTCTGTTGCCATACCA

 Occludin

F:TCATCGCCTCCATCGTCTAC

NM_205128.1

R:TCTTACTGCGCGTCTTCTGG

ZO-1

F: TATGAAGATCGTGCGCCTCC

XM_015278981.1

R: GAGGTCTGCCATCGTAGCTC

 Mucin-2

F: AGCGAGATGTTGGCGATGAT

NM_001318434.1

R: AAGTTGCCACACAGACCACA

Growth factors

TGF-β3

F:TGCGGCCAGATGAGCAT

NM_205454.1

R:TGCACATTCCTGCCACTGA

IGF-2

F: TGGCTCTGCTGGAAACCTAC

NM_001030342.2

R: ACTTGGCATGAGATGGCTTC

EGFR

F: ACCAGCCTGCAGAGAATGTA

NM_205497

R: CACCATGTTAAGCGCAATGA

GLP-2

F:AAGCTTCCCAGTCTGAACCA

NM_205260.4

R:ATCCTGAGCTCGTCTGCTGT

House-keeping genes

 β-actin

F: GAGAAATTGTGCGTGACATCA

NM 205518

R: CCTGAACCTCTCATTGCCA

  1. 1Primers were designed and synthesized by Sango Biotech (Shanghai) Co., Ltd. F: forward; R: reverse
  2. ZO-1 zonula occludens-1; EGFR epidermal growth factor receptor; GLP-2 glucagon-like peptide-2; IGF-2 insulin-like growth factor-2; TGF- β3 transforming growth factor beta 3