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Table 2 Nucleotide sequences of primers (TLR-mediated signaling pathway-related cytokines, chemokines and negative regulators) for quantitative real-time PCR1 assay

From: Dietary encapsulated essential oils and organic acids mixture improves gut health in broiler chickens challenged with necrotic enteritis

Name

Primer sequence (5' → 3')

GenBank accession

Receptors

TLR-2

F: GGGGCTCACAGGCAAAATC

NM_001161650.1

R: AGCAGGGTTCTCAGGTTCACA

TLR-4

F:CCACTATTCGGTTGGTGGAC

NM_001030693.1

R:ACAGCTTCTCAGCAGGCAAT

Adaptor proteins

MyD88

F:GGATGGTGGTCGTCATTTCA

NM_001030962.1

R:GAGATTTTGCCAGTCTTGTCCA

TRAF-6

F: CACAGAGGAGACGCAGGGATA

XM_001235884.1

R: AACAGATCGGGCACTCGTATTT

NF-kB

F:TGGAGAAGGCTATGCAGCTT

NM_205134.1

R:CATCCTGGACAGCAGTGAGA

Pro-inflammatory cytokines

TNFSF15

F- CCAAGAGCACACCTGACAGT

NM_001024578.1

R- CACAGGTATCACCAGTGCGT

IL-1β

F-CAGCAGCCTCAGCGAAGAG

NM_204524.1

R-CTGTGGTGTGCTCAGAATCCA

IL-8

F-GGCTTGCTAGGGGAAATGA

AJ009800

R-AGCTGACTCTGACTAGGAAACTGT

IFN-γ

F-AAAGCCGCACATCAAACACA

NM_205149.1

R-GCCATCAGGAAGGTTGTTTTTC

Ant-inflammatory cytokines

IL-10

F:CGCTGTCACCGCTTCTTCA

NM_001004414.2

R:CGTCTCCTTGATCTGCTTGATG

Negative regulators

Tollip

F:CATGGTACCTGTGGCAATACC

NM_001006471

R:GCACTGAGCGGATTACTTCC

PI3K

F:AACATCTGGCAAAACCAAGG

NM_001004410

R:CTGCAATGCTCCCTTTAAGC

A20

F:GAGAACGCAGAGCCTACACC

NM_001277522.1

R:CCAACCTTCTTCCTGCACAT

SOCS-1

F:GCTCTCAGGCTCGAGGTTAC

NM_001137648.1

R:GCTTGCTCGAGTGATGCTACT

SOCS-6

F: CAGATATCTTTGTGGACCAGGCAGTGAA

NM_001127312

R: GGTAGCAAAGGTGAAAGTGGAGGGACATC

  1. 1Primers were designed and synthesized by Sango Biotech (Shanghai) Co., Ltd. F: forward; R: reverse
  2. TLR Toll-like receptor; MyD88 myeloid differential protein-88; TRAF-6 TNF receptor-associated factor 6; NF-κB nuclear factor kappa-light-chain-enhancer of activated B cells; TNFSF15 tumor necrosis factor superfamily member 15; IL interleukin; IFN-γ interferon γ; Tollip Toll-interacting protein; PI3K phosphatidylinositol 3-kinase; A20 protein A20; SOCS suppressor of cytokine signaling