Table 2 Nucleotide sequences of primers (TLR-mediated signaling pathway-related cytokines, chemokines and negative regulators) for quantitative real-time PCR1 assay
Name | Primer sequence (5' → 3') | GenBank accession | |
|---|---|---|---|
Receptors | TLR-2 | F: GGGGCTCACAGGCAAAATC | NM_001161650.1 |
R: AGCAGGGTTCTCAGGTTCACA | |||
TLR-4 | F:CCACTATTCGGTTGGTGGAC | NM_001030693.1 | |
R:ACAGCTTCTCAGCAGGCAAT | |||
Adaptor proteins | MyD88 | F:GGATGGTGGTCGTCATTTCA | NM_001030962.1 |
R:GAGATTTTGCCAGTCTTGTCCA | |||
TRAF-6 | F: CACAGAGGAGACGCAGGGATA | XM_001235884.1 | |
R: AACAGATCGGGCACTCGTATTT | |||
NF-kB | F:TGGAGAAGGCTATGCAGCTT | NM_205134.1 | |
R:CATCCTGGACAGCAGTGAGA | |||
Pro-inflammatory cytokines | TNFSF15 | F- CCAAGAGCACACCTGACAGT | NM_001024578.1 |
R- CACAGGTATCACCAGTGCGT | |||
IL-1β | F-CAGCAGCCTCAGCGAAGAG | NM_204524.1 | |
R-CTGTGGTGTGCTCAGAATCCA | |||
IL-8 | F-GGCTTGCTAGGGGAAATGA | AJ009800 | |
R-AGCTGACTCTGACTAGGAAACTGT | |||
IFN-γ | F-AAAGCCGCACATCAAACACA | NM_205149.1 | |
R-GCCATCAGGAAGGTTGTTTTTC | |||
Ant-inflammatory cytokines | IL-10 | F:CGCTGTCACCGCTTCTTCA | NM_001004414.2 |
R:CGTCTCCTTGATCTGCTTGATG | |||
Negative regulators | Tollip | F:CATGGTACCTGTGGCAATACC | NM_001006471 |
R:GCACTGAGCGGATTACTTCC | |||
PI3K | F:AACATCTGGCAAAACCAAGG | NM_001004410 | |
R:CTGCAATGCTCCCTTTAAGC | |||
A20 | F:GAGAACGCAGAGCCTACACC | NM_001277522.1 | |
R:CCAACCTTCTTCCTGCACAT | |||
SOCS-1 | F:GCTCTCAGGCTCGAGGTTAC | NM_001137648.1 | |
R:GCTTGCTCGAGTGATGCTACT | |||
SOCS-6 | F: CAGATATCTTTGTGGACCAGGCAGTGAA | NM_001127312 | |
R: GGTAGCAAAGGTGAAAGTGGAGGGACATC | |||