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Table 2 Least square means of mucin staining intensity in the intestinal crypts of the broiler chickens in relation to diet, mucin type, gut segment and crypt fragment

From: Black soldier fly and gut health in broiler chickens: insights into the relationship between cecal microbiota and intestinal mucin composition

 

Factor

Factor levels

Mucin staining intensity1,2

Crypts

Diet

C

1.23 ± 0.03

HI5

1.27 ± 0.03

HI10

1.27 ± 0.03

HI15

1.30 ± 0.03

Mucin type

Neutral

1.32 ± 0.03A

Acidic sialylated

1.31 ± 0.02A

Acidic sulfated

1.18 ± 0.02B

Gut segment

Duodenum

1.26 ± 0.03B

Jejunum

1.20 ± 0.03BC

Ileum

1.45 ± 0.03A

Caecum

1.19 ± 0.03C

Fragment

Base

1.59 ± 0.03A

Midsection

1.17 ± 0.02B

Tip

1.10 ± 0.02C

Villi

Diet

C

1.90 ± 0.04A

HI5

1.87 ± 0.04A

HI10

1.72 ± 0.04B

HI15

1.66 ± 0.04B

Mucin type

Neutral

1.79 ± 0.04ab

Acidic sialylated

1.84 ± 0.04a

Acidic sulfated

1.74 ± 0.03b

Gut segment

Duodenum

1.23 ± 0.03C

Jejunum

1.95 ± 0.04B

Ileum

2.37 ± 0.04A

Fragment

Base

1.75 ± 0.03

Midsection

1.81 ± 0.04

Tip

1.80 ± 0.04

  1. C control, HI5 5% inclusion level of Hermetia illucens, HI10 10% inclusion level of Hermetia illucens, HI15 15% inclusion level of Hermetia illucens
  2. 1Data are represented as mean of counts ± SEM
  3. 2Means with different superscript letters (a, b or A, B, C) within the same column per predictor (i.e. diet, mucin type, gut segment or fragment) differ significantly (P < 0.05 or P < 0.01, respectively)