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Table 2 Least square means of mucin staining intensity in the intestinal crypts of the broiler chickens in relation to diet, mucin type, gut segment and crypt fragment

From: Black soldier fly and gut health in broiler chickens: insights into the relationship between cecal microbiota and intestinal mucin composition

 FactorFactor levelsMucin staining intensity1,2
CryptsDietC1.23 ± 0.03
HI51.27 ± 0.03
HI101.27 ± 0.03
HI151.30 ± 0.03
Mucin typeNeutral1.32 ± 0.03A
Acidic sialylated1.31 ± 0.02A
Acidic sulfated1.18 ± 0.02B
Gut segmentDuodenum1.26 ± 0.03B
Jejunum1.20 ± 0.03BC
Ileum1.45 ± 0.03A
Caecum1.19 ± 0.03C
FragmentBase1.59 ± 0.03A
Midsection1.17 ± 0.02B
Tip1.10 ± 0.02C
VilliDietC1.90 ± 0.04A
HI51.87 ± 0.04A
HI101.72 ± 0.04B
HI151.66 ± 0.04B
Mucin typeNeutral1.79 ± 0.04ab
Acidic sialylated1.84 ± 0.04a
Acidic sulfated1.74 ± 0.03b
Gut segmentDuodenum1.23 ± 0.03C
Jejunum1.95 ± 0.04B
Ileum2.37 ± 0.04A
FragmentBase1.75 ± 0.03
Midsection1.81 ± 0.04
Tip1.80 ± 0.04
  1. C control, HI5 5% inclusion level of Hermetia illucens, HI10 10% inclusion level of Hermetia illucens, HI15 15% inclusion level of Hermetia illucens
  2. 1Data are represented as mean of counts ± SEM
  3. 2Means with different superscript letters (a, b or A, B, C) within the same column per predictor (i.e. diet, mucin type, gut segment or fragment) differ significantly (P < 0.05 or P < 0.01, respectively)