Table 1 Primers for gene expression analysis using real-time PCR
Gene name | Primer sequences (5′→3′) | GenBank accession | Expected size, bp |
|---|---|---|---|
ZO1 | F: CCACTGCCTACACCACCATCTC R: CGTGTCACTGGGGTCCTTCAT | XM_015278975.1 | 138 |
ZO2 | F: CCCAGTGGTTTCCCATTGTAGTC R: GAACACAGCCTTTGTCTCATCGT | XM_015280242.1 | 79 |
CLDN1 | F: GCATGGAGGATGACCAGGTGA R: GAGCCACTCTGTTGCCATACCAT | NM_001013611.2 | 117 |
CLDN2 | F: CCTACATTGGTTCAAGCATCGTGA R: GATGTCGGGAGGCAGGTTGA | NM_001277622.1 | 131 |
GAPDH | F: CAGAACATCATCCCAGCGTCCA R: ACGGCAGGTCAGGTCAACAA | NM_204305.1 | 135 |