Table 3 The DEGs and biological pathways regulated in treatment groups (Control vs. 39 °C, Control vs. 40 °C, Control vs. 41 °C)
Pathway name | Gene count | P-value | Gene name |
|---|---|---|---|
Control vs. 39 °C | |||
 Protein processing in endoplasmic reticulum | 13 | 1.23E-07 | HSP90AB1, TRAF2, HSPH1, ATF4, HSP90B1, HSP90AA1, RNF5, BAX, RNF185, EDEM2, EIF2AK4, HSPA8, BCAP31 |
 FoxO signaling pathway | 9 | 5.74E-05 | PLK1, CCND2, MDM2, CAT, FOXO3, G6PC3, CDK2, SOD2 |
 Pathways in cancer | 13 | 7.19E-04 | HSP90AB1, TRAF2, CASP3, HSP90B1, HSP90AA1, BAX, GNB5, MDM2, BCL2L1, NFKB2, FGF12, CRK, CDK2 |
 PI3K-Akt signaling pathway | 12 | 7.77E-04 | HSP90AB1, ATF4, HSP90B1, HSP90AA1, CCND2, GNB5, MDM2, FOXO3, BCL2L1, FGF12, G6PC3, CDK2 |
 Antigen processing and presentation | 6 | 9.96E-04 | HSP90AB1, HSP90AA1, NFYC, IFI30, HSPA4, HSPA8 |
 bta05215:Prostate cancer | 6 | 1.80E-03 | HSP90AB1, ATF4, HSP90B1, HSP90AA1, MDM2, CDK2 |
 Amyotrophic lateral sclerosis (ALS) | 5 | 2.20E-03 | CASP3, BAX, CAT, BCL2L1, SOD1 |
 Apoptosis | 5 | 3.70E-03 | CASP6, TRAF2, CASP3, BAX, BCL2L1 |
 MAPK signaling pathway | 9 | 4.70E-03 | TRAF2, CASP3, ATF4, MAPK8IP1, NFKB2, FGF12, CRK, HSPA8, PPP5C |
 p53 signaling pathway | 5 | 6.00E-03 | CASP3, CCND2, BAX, MDM2, CDK2 |
 Aldosterone synthesis and secretion | 5 | 8.70E-03 | ATF4, CYP11A1, STAR, SCARB1, ATF1 |
 Viral carcinogenesis | 8 | 0.010 | TRAF2, CASP3, ATF4, CCND2, BAX, MDM2, NFKB2, CDK2 |
 Ovarian steroidogenesis | 4 | 0.014 | CYP11A1, STAR, SCARB1, BMP6 |
 Estrogen signaling pathway | 5 | 0.018 | HSP90AB1, ATF4, HSP90B1, HSP90AA1, HSPA8 |
 Legionellosis | 4 | 0.020 | CASP3, HSPD1, NFKB2, HSPA8 |
 Vitamin digestion and absorption | 3 | 0.021 | BTD, ABCC1, SCARB1 |
 Cell cycle | 5 | 0.039 | PLK1, CCND2, PCNA, MDM2, CDK2 |
 Epstein-Barr virus infection | 6 | 0.047 | TRAF2, MDM2, NFKB2, CDK2, EIF2AK4, HSPA8 |
Control vs. 40 °C | |||
 Ribosome | 11 | 2.18E-04 | RPS26, MRPL23, MRPL21, MRPL15, RPLP0, RPL15, MRPL36, RPS11, MRPL33, UBA52, RPS24 |
 Protein processing in endoplasmic reticulum | 12 | 3.51E-04 | HSP90AB1, TRAF2, HSPH1, HSP90B1, HSP90AA1, EIF2AK1, RNF5, BAX, RNF185, EDEM2, HSPA8, BCAP31 |
 FoxO signaling pathway | 10 | 8.75E-04 | HRAS, PLK1, CCND2, PRKAB1, CAT, FOXO3, CCNG2, CDK2, SOD2 |
 p53 signaling pathway | 7 | 0.002 | CASP3, CCND2, BAX, TSC2, RPRM, CCNG2, CDK2 |
 Antigen processing and presentation | 7 | 0.002 | HSP90AB1, CTSL, HSP90AA1, NFYC, HSPA4, RFXANK, HSPA8 |
 Amyotrophic lateral sclerosis (ALS) | 6 | 0.003 | CASP3, BAX, TOMM40L, CAT, BCL2L1, SOD1 |
 Biosynthesis of antibiotics | 10 | 0.016 | SC5D, UAP1, IDH3G, PAFAH2, PGM1, RCE1, CAT, UAP1L1, PAICS, OAT |
 Base excision repair | 4 | 0.024 | LIG3, PCNA, OGG1, APEX1 |
 Glutathione metabolism | 3 | 0.026 | GSS, GSR, GSTA3 |
 Apoptosis | 5 | 0.029 | CASP6, TRAF2, CASP3, BAX, BCL2L1 |
 PI3K-Akt signaling pathway | 12 | 0.035 | HSP90AB1, HRAS, HSP90B1, HSP90AA1, CCND2, TSC2, GNG2, FOXO3, BCL2L1, FGF12, CDK2, GHR |
 Ovarian steroidogenesis | 4 | 0.038 | CYP11A1, STAR, SCARB1, BMP6 |
Control vs 41 °C | |||
 Protein processing in endoplasmic reticulum | 14 | 1.40E-05 | HSP90AB1, TRAF2, HSP90AA1, RNF185, EDEM2, BCAP31, HSPH1, HSP90B1, EIF2AK1, DNAJB11, RNF5, BAX, EIF2AK2, HSPA8 |
 Viral carcinogenesis | 14 | 3.92E-04 | TRAF2, CASP3, HDAC1, CCND2, DDB1, BAX, CREBBP, RHOA, PIK3CA, NFKB2, EIF2AK2, SRF, CDK2, SCRIB |
 Biosynthesis of unsaturated fatty acids | 5 | 9.11E-04 | PECR, ACOT7, ELOVL5, HACD2, FADS2 |
 Pathways in cancer | 18 | 0.001 | HSP90AB1, DVL2, TRAF2, HSP90AA1, EPAS1, CREBBP, BCL2L1, KIT, NFKB2, CDK2, VEGFC, FOS, CASP3, HSP90B1, HDAC1, BAX, RHOA, PIK3CA |
 HTLV-I infection | 14 | 0.001 | DVL2, TSPO, MRAS, CREBBP, NFKB2, BCL2L1, SRF, FOS, CCND2, BAX, PCNA, RRAS, PIK3CA, NFATC3 |
 FoxO signaling pathway | 9 | 0.003 | PLK1, CCND2, CREBBP, PIK3CA, CAT, FOXO3, CDK2, SOD2 |
 Huntington's disease | 9 | 0.003 | CASP3, HDAC1, BAX, CREBBP, NDUFV2, NDUFS3, SOD1, SOD2 |
 Peroxisome | 6 | 0.004 | PECR, PEX2, MPV17, CAT, SOD1, SOD2 |
 Apoptosis | 6 | 0.005 | CASP6, TRAF2, CASP3, BAX, PIK3CA, BCL2L1 |
 Hepatitis B | 9 | 0.006 | FOS, CASP3, DDB1, BAX, CREBBP, PCNA, PIK3CA, NFATC3, CDK2 |
 p53 signaling pathway | 6 | 0.010 | CASP3, CCND2, CD82, BAX, CDK2 |
 Fatty acid metabolism | 5 | 0.011 | PECR, ELOVL5, HACD2, FADS2, HADH |
 Fatty acid elongation | 4 | 0.011 | ACOT7, ELOVL5, HACD2, HADH |
 Cell cycle | 6 | 0.013 | HDAC1, PLK1, CCND2, CREBBP, PCNA, CDK2 |
 Ovarian steroidogenesis | 4 | 0.015 | CYP11A1, STAR, SCARB1, BMP6 |
 Amyotrophic lateral sclerosis (ALS) | 5 | 0.017 | CASP3, BAX, CAT, BCL2L1, SOD1 |
 TGF-beta signaling pathway | 6 | 0.018 | INHBB, INHBA, CREBBP, RHOA, TGIF2, BMP6 |
 MAPK signaling pathway | 11 | 0.021 | TRAF2, FOS, CASP3, MRAS, PTPRR, RRAS, MAPK8IP1, NFKB2, NFATC3, SRF, HSPA8 |
 Prostate cancer | 6 | 0.021 | HSP90AB1, HSP90B1, HSP90AA1, CREBBP, PIK3CA, CDK2 |
 Epstein-Barr virus infection | 9 | 0.026 | TRAF2, EIF2AK1, HDAC1, CREBBP, PIK3CA, NFKB2, EIF2AK2, CDK2, HSPA8 |
 Colorectal cancer | 5 | 0.0339 | FOS, CASP3, BAX, RHOA, PIK3CA |
 Estrogen signaling pathway | 6 | 0.0354 | HSP90AB1, FOS, HSP90B1, HSP90AA1, PIK3CA, HSPA8 |
 Glutathione metabolism | 3 | 0.0386 | GSR, GSTA3, G6PD |