Fig. 5
Heat stress enhanced intracellular ROS accumulation in bovine granulosa cells: Fluorescent photomicrographs of bovine granulosa cells (bGCs) stained with 2′,7′-dichlorofluorescin diacetate (H2DCFDA) were shown control (38 °C) (a) and heat stress (39, 40 and 41 °C) (b, c, d, respectively). The images shown are representative of the three independent image acquisitions. e Quantitative analysis of relative fluorescence emission. Values are expressed as mean ± SEM of n = 3. Superscripts (a, b, c) show significant difference, P < 0.05