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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Evaluation of heat stress effects on cellular and transcriptional adaptation of bovine granulosa cells

Fig. 3

Heat stress exposure elevates bovine granulosa cell apoptosis and decreases viability: Flow cytometric analysis of bovine granulosa cells (bGCs) cultured under heat stress (39, 40 and 41 °C) and corresponding control (38 °C) (a, b). The analyzed cell counts for apoptosis and viability are indicated on the Y-axis and the temperature treatments are indicated on the X-axis. Data shown as means ± SEM, n = 3, P < 0.05. Fluorescent photomicrographs of bovine granulosa cells (bGCs) stained with 2′,7′-dichlorofluorescin diacetate (H2DCFDA) were shown control (38 °C) (c) and heat stress (39, 40 and 41 °C) (d, e, f, respectively). The images shown are representative of the three independent image acquisitions. g Quantitative analysis of relative fluorescence emission. Values are expressed as mean ± SEM of n = 3. Superscripts (a, b, c) show significant difference, P < 0.05

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