Fig. 3

Impaired endometrial remodeling and dysregulated redox homeostasis in IVF endometrial C areas. a Classification of GO terms based on functional annotation of ‘biological process’, ‘cellular component’, and ‘molecular function’, using DEPs between IVO and IVF C areas. The left ordinate represents the number of DEPs enriched in each term [defined as log₂ (No. of enriched genes)], and the right ordinate represents the enrichment score [defined as –log₁₀(P-value)]. b Heat map of DEPs associated with mitochondrial metabolism and translation in the IVO and IVF C areas. Normalized protein abundance is represented in red (relatively high) and green (relatively low). c Normalized abundance of proteins involved in cellular proliferation in IVO and IVF C areas. d Comparisons of the total abundance of 1548 proteins of the IVO and IVF C area samples. Each circle indicates the total abundance of 1548 proteins of a biological replicate from the IVO or IVF C area samples. e Quantitation of total protein concentration per gram of tissue in IVO and IVF C area samples. Data represent the mean ± SEM of three independent biological replicates, *P < 0.05. f Normalized abundance of proteins encoded by interferon-induced genes in the IVO and IVF C areas. Data represent the mean ± SEM, * P < 0.05. g (Right) Heat map of DEPs associated with cell redox homeostasis in the IVO and IVF C areas. Normalized protein abundance is represented in red (relatively high) and green (relatively low). (Left) Normalized abundance of proteins involved in cellular homeostasis in the IVO and IVF C areas. h Representative fluorescent images of cell nucleus stained by DAPI (blue) and the cytoskeletal structure stained by phalloidin (green) in human endometrial cancer cells (Ishikawa line) following different treatments