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Table 2 Chemical composition of mucosal and serosal buffers used in the Ussing chamber study

From: Effects of dietary menthol-rich bioactive lipid compounds on zootechnical traits, blood variables and gastrointestinal function in growing sheep

Item T&S solution Mucosal solutions, rumen Mucosal solutions, intestine
Na+-containing Na+-free Na+-containing Na+-free
Composition, mmol/L
 CaCl2 1.8 1.8 1.8 1.8 1.8
 MgCl2 1.0 0 0 0 0
 HEPES, free acid 10.0 10.0a 10.0a 10.0 10.0
 NaCl 70.0 70.0 0.0 70.0 0.0
 HCl 0.0 17.0 87.0 2.0 72.0
 Na-acetate 0.0 25.0 0.0 0.0 0.0
 Acetic acid 0.0 0.0 25.0 0.0 0.0
 Na-propionate 0.0 10.0 0.0 0.0 0.0
 Propionic acid 0.0 0.0 10.0 0.0 0.0
 Na-butyrate 0.0 5.0 0.0 0.0 0.0
 Butyric acid 0.0 0.0 5.0 0.0 0.0
 Na-gluconate 40.0 0.0 0.0 40.0 0.0
 Gluconic acid 0.0 0.0 0.0 0.0 40.0
 NMDG free base 5.0 2.0 112 7.0 117
 KH2PO4 0.4 0.4 0.4 0.4 0.4
 K2HPO4 2.4 2.4 2.4 2.4 2.4
 NaHCO3 25.0 25.0 0.0 25.0 0.0
 Cholin- HCO3 0.0 0.0 25.0 0.0 25.0
 Enrofloxacine 0.0278 0.0278 0.0278 0.0278 0.0278
 Mannitol 0 19.0 19.0 9.0 9.0
 Glucose 10.0 0 0 0 0
Osmolarity, mOsmol/L 288 288 288 288 288
pH 7.4 6.4 6.4 7.4 7.4
  1. HEPES: 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid; NMDG: N-methyl-D-glucamine; T&S: Transport and serosal
  2. aRuminal mucosal buffer contained MES (2-morpholinoethanesulfonic acid) free acid instead of HEPES free acid