Table 2 Chemical composition of mucosal and serosal buffers used in the Ussing chamber study
Item | T&S solution | Mucosal solutions, rumen | Mucosal solutions, intestine | ||
|---|---|---|---|---|---|
Na+-containing | Na+-free | Na+-containing | Na+-free | ||
Composition, mmol/L | |||||
 CaCl2 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 |
 MgCl2 | 1.0 | 0 | 0 | 0 | 0 |
 HEPES, free acid | 10.0 | 10.0a | 10.0a | 10.0 | 10.0 |
 NaCl | 70.0 | 70.0 | 0.0 | 70.0 | 0.0 |
 HCl | 0.0 | 17.0 | 87.0 | 2.0 | 72.0 |
 Na-acetate | 0.0 | 25.0 | 0.0 | 0.0 | 0.0 |
 Acetic acid | 0.0 | 0.0 | 25.0 | 0.0 | 0.0 |
 Na-propionate | 0.0 | 10.0 | 0.0 | 0.0 | 0.0 |
 Propionic acid | 0.0 | 0.0 | 10.0 | 0.0 | 0.0 |
 Na-butyrate | 0.0 | 5.0 | 0.0 | 0.0 | 0.0 |
 Butyric acid | 0.0 | 0.0 | 5.0 | 0.0 | 0.0 |
 Na-gluconate | 40.0 | 0.0 | 0.0 | 40.0 | 0.0 |
 Gluconic acid | 0.0 | 0.0 | 0.0 | 0.0 | 40.0 |
 NMDG free base | 5.0 | 2.0 | 112 | 7.0 | 117 |
 KH2PO4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 |
 K2HPO4 | 2.4 | 2.4 | 2.4 | 2.4 | 2.4 |
 NaHCO3 | 25.0 | 25.0 | 0.0 | 25.0 | 0.0 |
 Cholin- HCO3 | 0.0 | 0.0 | 25.0 | 0.0 | 25.0 |
 Enrofloxacine | 0.0278 | 0.0278 | 0.0278 | 0.0278 | 0.0278 |
 Mannitol | 0 | 19.0 | 19.0 | 9.0 | 9.0 |
 Glucose | 10.0 | 0 | 0 | 0 | 0 |
Osmolarity, mOsmol/L | 288 | 288 | 288 | 288 | 288 |
pH | 7.4 | 6.4 | 6.4 | 7.4 | 7.4 |