Table 1 Nucleotide sequences of specific primers used for real-time PCR
Target genes | GenBank accession | Primer sequences (5′ to 3′) | PCR products, bp |
|---|---|---|---|
StAR | NM_204686.2 | F:ATGGCATCCAAGGAGTGA | 103 |
R: GGGAGACAGAAGGGAACAG | |||
CYP11A1 | NM_001001756.1 | F: AGCACTTCAAGGGACTGAGC | 147 |
R:ACTTGGTCCCAACTTCCACC | |||
3BHSD2 | XM_417988.5 | F: CTGGAAGAAGATGAGGCGCT | 249 |
R: ACCTGTCACGTTGACTTCCC | |||
CYP21A2 | >NM_001099358.1 | F: CTTTGAGGCGTTCACGTTCC | 169 |
R: CTGGGACTCCACAAAGGCAT | |||
CYP19A1 | NM_001364699.1 | F: CATGCACCCAATAGAAAGGCA | 130 |
R: GCATTTCTTAAAGTGACTGCAAAC | |||
CYP17A1 | NM_001001901.2 | F: TCTGCTCCCTCTGCTTCAA | 250 |
R: AGGTCCCTCACAGTGTCCC | |||
SF-1 | XM_015279334.1 | F: TCTTCCTGAATTTCCCTT | 151 |
R: TGAACATCCCATCTAGTGA | |||
CREB | XM_015294628.2 | F: GTCAGACACACCAGAGCCTT | 128 |
R: CATTCCTGCTCCCCTTCCTC | |||
BHMT | XM_414685.3 | F: CGAGTGGGACGGCTTCTT | 144 |
R: AGGCGATAGGTGTCAGGGA | |||
AHCYL1 | NM_001030913.1 | F:TGGTGTTGTTGGGGGAGAT | 227 |
R:CCCCATCAATACTCATCCAAC | |||
DNMT1 | NM_206952.1 | F:TGATAYGTTGGATGAGTATTGATGG | 264 |
R: AAAAAAACTCTCACTCAACTCCAC | |||
GNMT1 | XM_015283546.1 | F: GGAGGAGGGCTTCCAAGTGA | 140 |
R: GCTCCAGCGTCAGCCAGTT | |||
VIM | AH002482.2 | F: CTTTGCCCAGTGCTGTAGTC | 171 |
R: AAACACGGGCTGTCAGTCA | |||
VDAC1 | NM_001033869.2 | F: GAACAGGGACGGGGACAG | 136 |
R: CAGACTTGCCCAGATCAGCA |