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Table 3 Clinical data on pregnancy rate of cows inseminated with semen straw containing sperm cells selected prior to cryopreservation

From: Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility

Cow History of failed reproduction Sire Pregnancy
Heifer Japanese Black #1 None Bull #1 Yes
Japanese Black #2 None Bull #2 Yes
Japanese Black #3 None Bull #1 Yes
Japanese Black #4 None Bull #2 Yes
Repeat breeder Holstein #1 2 × ET, 2 × AI Bull #1 Yes
Holstein #2 2 × ET, 3 × AI Bull #2 Yes
Holstein #3 2 × ET, 2 × AI Bull #1 Yes
Holstein #4 2 × AI Bull #2 No
Holstein #4 3 × AI Bull #1 Yes
Holstein #5 2 × ET, 3 × AI Bull #1 Yes
Holstein #6 4 × AI Bull #1 Yes
Holstein #7 2 × ET, 2 × AI Bull #2 Yes
Holstein #8 4 × AI Bull #2 Yes
  1. ET embryo transfer, AI artificial insemination using conventional frozen semen
  2. AI for Holstein #4 had been performed twice; first AI resulted in nonpregnancy, second AI achieved pregnancy
  3. Pregnancy rates of Japanese Black and Holstein cows (n = 12) inseminated with pre-freeze selected sperm cells: Bull #1 = 100%, n = 7; Bull #2 = 83.3%, n = 6, Total pregnancy rate = 92.3%
  4. Pregnancy rates of control Japanese Black cows inseminated with conventionally prepared semen from the 2 sires; Bull #1 = 59.2%, n = 76; Bull #2 = 44.4%, n = 36, Total pregnancy rate = 54.5%
  5. Values between SSTR and conventional straws differ significantly at P < 0.01 (Chi-squared test)