Table 3 Sperm quality parameters from samples exposed to extender alone (control), or in the presence of phloretin (PHL) at three different concentrations (250 μmol/L, 500 μmol/L and 1000 μmol/L). The evaluated parameters were: percentage of progressively motile spermatozoa (PMOT), percentage of spermatozoa with low membrane lipid disorder (%M540−/YO-PRO-1− sperm cells) and percentage of spermatozoa with high mitochondrial membrane potential (MMP; %JC1agg spermatozoa). Determinations were performed in fresh (extended) and frozen-thawed (FT) spermatozoa after 30 and 240 min of thawing. Data reported as mean ± SEM
From: Aquaglyceroporins but not orthodox aquaporins are involved in the cryotolerance of pig spermatozoa
Variable | PHL concentration | Time point | ||
|---|---|---|---|---|
Extended | FT-30 min | FT-240 min | ||
%PMOT | CNT | 69.87 ± 3.20 | 23.38 ± 2.74a | 10.96 ± 1.12a |
250 μmol/L | 20.52 ± 0.98a | 8.61 ± 0.93a | ||
500 μmol/L | 22.16 ± 0.75a | 7.62 ± 0.89a | ||
1000 μmol/L | 10.38 ± 0.90b | 3.82 ± 0.92b | ||
%M540−/ YO-PRO-1− | CNT | 80.83 ± 2.79 | 39.03 ± 1.49a, b | 31.25 ± 1.06a |
250 μmol/L | 44.53 ± 1.99a | 34.19 ± 1.29a | ||
500 μmol/L | 37.92 ± 1.37b | 30.20 ± 1.10a | ||
1000 μmol/L | 31.01 ± 1.60c | 21.42 ± 1.05b | ||
%JC1agg | CNT | 82.79 ± 2.41 | 31.95 ± 1.89a | 26.01 ± 1.44a |
250 μmol/L | 38.21 ± 2.08a | 22.49 ± 1.21a | ||
500 μmol/L | 34.98 ± 2.09a | 29.29 ± 1.74a | ||
1000 μmol/L | 31.93 ± 1.88a | 25.18 ± 1.29a | ||