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Table 2 Sperm quality parameters from samples exposed to extender alone (control), or in the presence of acetazolamide (AC) at three different concentrations (250 μmol/L, 500 μmol/L and 1000 μmol/L). The evaluated parameters were: percentage of progressively motile spermatozoa (PMOT), percentage of spermatozoa with low membrane lipid disorder (%M540/YO-PRO-1 sperm cells) and percentage of spermatozoa with high mitochondrial membrane potential (MMP; %JC1agg spermatozoa). Determinations were performed in fresh (extended) and frozen-thawed (FT) spermatozoa after 30 and 240 min of thawing. Data reported as mean ± SEM

From: Aquaglyceroporins but not orthodox aquaporins are involved in the cryotolerance of pig spermatozoa

Variable

AC concentration

Time point

Extended

FT-30 min

FT-240 min

%PMOT

CNT

69.87 ± 3.20

23.38 ± 2.74a

10.96 ± 1.12a, b

250 μmol/L

25.34 ± 2.83a

13.47 ± 2.19a

500 μmol/L

22.99 ± 2.57a

12.94 ± 1.98a, b

1000 μmol/L

20.77 ± 2.29a

8.93 ± 1.30b

%M540/ YO-PRO-1

CNT

80.83 ± 2.79

39.03 ± 1.49a, b

31.25 ± 1.06a, b

250 μmol/L

40.26 ± 1.73a, b

27.01 ± 0.98a

500 μmol/L

46.39 ± 1.30a

35.28 ± 1.71b

1000 μmol/L

36.03 ± 1.58b

30.52 ± 1.18a, b

%JC1agg

CNT

82.79 ± 2.41

31.95 ± 1.89a

26.01 ± 1.44a, b

250 μmol/L

29.30 ± 1.63a

23.70 ± 1.24a, b

500 μmol/L

40.84 ± 2.11b

31.98 ± 1.89a

1000 μmol/L

30.20 ± 1.72a

21.92 ± 1.35b

  1. Different letters (a, b) indicate significant differences (P < 0.05) between treatments within a given time point for each parameter