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Table 1 Sperm quality parameters from samples exposed to extender alone (control), or in the presence of 1,3-propanediol (PDO) at three different concentrations (0.1 mmol/L, 1 mmol/L and 10 mmol/L). The evaluated parameters were: percentage of progressively motile spermatozoa (PMOT), percentage of spermatozoa with low membrane lipid disorder (%M540/YO-PRO-1 sperm cells) and percentage of spermatozoa with high mitochondrial membrane potential (MMP; %JC1agg spermatozoa). Determinations were performed in fresh (extended) and frozen-thawed (FT) spermatozoa after 30 and 240 min of thawing. Data reported as mean ± SEM

From: Aquaglyceroporins but not orthodox aquaporins are involved in the cryotolerance of pig spermatozoa

Variable

PDO concentration

Time point

Extended

FT-30 min

FT-240 min

%PMOT

CNT

69.87 ± 3.20

23.38 ± 2.74a

10.96 ± 1.12a,b

0.1 mmol/L

26.62 ± 2.10a

7.60 ± 0.42b

1 mmol/L

22.80 ± 2.12a

11.71 ± 1.07a

10 mmol/L

24.94 ± 2.20a

8.48 ± 0.45a,b

%M540/ YO-PRO-1

CNT

80.83 ± 2.79

39.03 ± 1.49a

31.25 ± 1.06a

0.1 mmol/L

48.12 ± 1.75b

37.56 ± 1.50b,c

1 mmol/L

53.28 ± 2.10b

42.93 ± 1.38c

10 mmol/L

56.02 ± 2.32b

36.50 ± 1.23a,b

%JC1agg

CNT

82.79 ± 2.41

31.95 ± 1.89a

26.01 ± 1.44a

0.1 mmol/L

48.76 ± 1.85b

38.67 ± 1.58b

1 mmol/L

40.95 ± 2.07c

34.39 ± 1.54b, c

10 mmol/L

35.89 ± 2.06a, c

30.56 ± 1.29a, c

  1. Different letters (a–c) indicate significant differences (P < 0.05) between treatments within a given time point for each parameter