Table 5 Primers used for quantitative real-time PCR
Genes | Accession number | Forward sequence (5′ to 3′) | Reverse sequence (5′ to 3′) | Role |
|---|---|---|---|---|
β-actin | NM_205518 | GAGAAATTGTGCGTGACATCA | CCTGAACCTCTCATTGCCA | Highly conserved, selected as the standard in the gene expression analysis |
GLUT-2 | Z22932 | CCGCAGAAGGTGATAGAAGC | ATTGTCCCTGGAGGTGTT | Glucose transporter |
SGLT-1 | XM_415247 | AGATTTGGAGGGCAGAGGAT | GCCCAAAGAGATTTGGATGA | Sodium dependant glucose transporter |
PepT-1 | AY029615 | TACGCATACTGTCACCATCA | TCCTGAGAACGGACTGTAAT | Di- and tri-peptide transporter |