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Table 8 Multiple regression analysis to choose the most important protein spectral parameters to predict protein supply using the NRC model

From: Interactive association between processing induced molecular structure changes and nutrient delivery on a molecular basis, revealed by cutting-edge vibrational biomolecular spectroscopy

Predicted variable, Y

Variable selection (variables left in the model with P < 0.05)

Equation prediction: Y = a + b1 × x1 + b2 × x2 + … … .

Model

R2

RSDa

P value

Absorbable microbial protein synthesis in the rumen (AMCP)b

 MCPRDP

Amide I height, β-sheet height

Y = 106.55–703.13 × Amide I height + 1008.90 × β-sheet height

0.67

11.507

< 0.01

 MCPTDN

Amide I height, β-sheet height

Y = 87.07 + 287.78 × Amide I height – 272.73 × β-sheet height

0.82

1.470

< 0.01

 AMCP

Amide I height, β-sheet height

Y = 55.72 + 184.17 × Amide I height – 174.54 × β-sheet height

0.82

0.941

< 0.01

Truly absorbable rumen-the undegraded protein in the small intestine (ARUP)c

 RUP

Amide I, II ratio

Y = −132.49 – 142.47 × Amide I, II ratio

0.34

28.926

< 0.01

 ARUP

Amide I, II ratio

Y = −165.71 – 138.71 × Amide I, II ratio

0.38

26.039

< 0.01

Total metabolizable protein (MP) and degraded protein balance (DPB)d

 MP

Amide I, II ratio

Y = −110.83 – 142.07 × Amide I, II ratio

0.37

27.278

< 0.01

 DPB

Amide I height, β-sheet height

Y = 22.61–1166.80 × Amide I height + 1508.67 × β-sheet height

0.74

13.358

< 0.01

Feed milk values based on metabolic characteristics of protein predicted by NRC systeme

 FMV

Amide I, II ratio

Y = −1.62 + 2.45 × Amide I, II ratio

0.20

0.710

< 0.01

  1. a RSD, residual standard deviation
  2. bMCPRDP, a microbial protein synthesized in the rumen based on available protein calculated as 0.85 of rumen-degraded protein; MCPTDN, a microbial protein synthesized in the rumen based on available energy (discounted TDN); AMCP, truly absorbed rumen-synthesized microbial protein in the small intestine
  3. c RUP, ruminally undegraded feed CP, calculated according to the formula in NRC–2001 dairy model; ARUP, truly absorbed rumen-undegraded feed protein in the small intestine
  4. d MP, metabolizable protein (a true protein that is digested postruminally and the component amino acid absorbed by the intestine); DPB, reflects the difference between the potential microbial protein synthesis based on ruminally degraded feed CP and that based on energy-TDN available for microbial fermentation in the rumen
  5. e FMV, feed milk value