Interactive association between processing induced molecular structure changes and nutrient delivery on a molecular basis, revealed by cutting-edge vibrational biomolecular spectroscopy

Table 5 Correlation between molecular structure related to amide region and predicted protein supply form combined feed (BPP; carinata meal and canola meal) using NRC system

Items		Amide I height	Amide II height	Amide I, II ratio	Amide I area	Amide II area	Amide area	α-helix height	β-sheet height	α-helix, β-sheet ratio
Absorbable microbial protein synthesis in the rumen, AMCP^a
MCP_RDP	r	0.64	0.68	−0.25	0.58	0.62	0.65	0.76	0.78	−0.60
MCP_RDP	P value	< 0.01	< 0.01	0.16	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01
MCP_TDN	r	−0.38	−0.53	0.23	−0.33	− 0.50	− 0.43	− 0.59	−0.63	0.55
MCP_TDN	P value	0.03	< 0.01	0.22	0.06	< 0.01	0.02	< 0.01	< 0.01	< 0.01
AMCP	r	−0.38	−0.53	0.23	−0.33	− 0.50	− 0.43	− 0.59	−0.63	0.55
AMCP	P value	0.03	< 0.01	0.22	0.06	< 0.01	0.02	< 0.01	< 0.01	< 0.01
Truly absorbable rumen–the undegraded protein in the small intestine, ARUP^b
RUP	r	0.17	−0.33	0.59	0.37	−0.24	0.19	−0.08	− 0.03	− 0.20
RUP	P value	0.35	0.07	< 0.01	0.04	0.19	0.29	0.66	0.86	0.27
ARUP	r	0.11	−0.41	0.62	0.30	−0.31	0.12	−0.15	− 0.11	−0.12
ARUP	P value	0.54	0.02	< 0.01	0.09	0.09	0.50	0.41	0.55	0.50
Total metabolizable protein (MP) and degraded protein balance, DPB^c
MP	r	0.08	−0.43	0.61	0.27	− 0.32	0.09	− 0.18	− 0.15	−0.09
MP	P value	0.65	0.02	< 0.01	0.14	0.07	0.62	0.32	0.43	0.64
DPB	r	0.63	0.70	−0.26	0.57	0.64	0.65	0.77	0.79	−0.62
DPB	P value	< 0.01	< 0.01	0.15	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01
Feed milk values based on metabolic characteristics of protein predicted by NRC system^d
FMV	r	−0.02	−0.39	0.45	0.15	−0.30	0.01	−0.25	−0.23	0.02
FMV	P value	0.91	0.03	0.01	0.43	0.10	0.97	0.17	0.21	0.90

^aMCP_RDP, a microbial protein synthesized in the rumen based on available protein calculated as 0.85 of rumen-degraded protein; MCP_TDN, a microbial protein synthesized in the rumen based on available energy (discounted TDN); AMCP, truly absorbed rumen-synthesized microbial protein in the small intestine
^bRUP, ruminally undegraded feed CP, calculated according to the formula in NRC–2001 dairy model; ARUP, truly absorbed rumen–undegraded feed protein in the small intestine
^cMP, metabolizable protein (a true protein that is digested postruminally and the component amino acid absorbed by the intestine); DPB, reflects the difference between the potential microbial protein synthesis based on ruminally degraded feed CP and that based on energy-TDN available for microbial fermentation in the rumen
^dFMV, feed milk value

ISSN: 2049-1891