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Table 2. Protein spectral profile (protein primary structures and protein secondary structures) of different blend pelleted products (BPP) of carinata meal and canola meal in the different levels of pea screenings and lignosulfonate and comparison between carinata meal and canola meal (CR vs. CN), adding lignosulfonate with no adding lignosulfonate (LSO3 No vs. Add), and high level of pea screenings with low level of pea screenings (Pea High vs. Low) using FTIR Vibrational Spectroscopy

From: Interactive association between processing induced molecular structure changes and nutrient delivery on a molecular basis, revealed by cutting-edge vibrational biomolecular spectroscopy

CO-P

Pea

LSO3

Treatments1

Amide I peak height4

Amide II peak height

Amide I, II height ratio

Amide I area

Amide II area

Amide area

α-helix height

β-sheet height

α-helix, β-sheet height ratio

CR

High

NO

BPP1 (n =2)

0.279cd

0.134bc

2.067bc

19.024d

6.469bc

25.494de

0.319cd

0.274de

1.166

CR

High

Add

BPP2 (n =2)

0.252e

0.123d

2.028bc

17.507e

5.760d

23.269f

0.285e

0.245f

1.167

CR

Low

NO

BPP3 (n =2)

0.302ab

0.140b

2.152ab

21.253a

6.532b

27.784ab

0.341b

0.299bc

1.141

CR

Low

Add

BPP4 (n =2)

0.281c

0.126cd

2.218a

20.008c

6.008cd

26.017cd

0.319cd

0.281cde

1.137

CN

High

NO

BPP5 (n =2)

0.279cd

0.143b

1.957cd

19.032d

6.408bc

25.441de

0.331bc

0.288cd

1.151

CN

High

Add

BPP6 (n =2)

0.265de

0.140b

1.934cd

18.008e

6.554b

24.562e

0.313d

0.266e

1.185

CN

Low

NO

BPP7 (n =2)

0.312a

0.164a

1.892d

21.090ab

7.642a

28.732a

0.374a

0.334a

1.120

CN

Low

Add

BPP8 (n =2)

0.288bc

0.138b

2.139ab

20.225bc

6.605b

26.827bc

0.345b

0.311b

1.110

SEM2

0.0058

0.0041

0.0692

0.3357

0.2136

0.429

0.0067

0.0073

0.0201

P value

<0.01

<0.01

<0.01

<0.01

<0.01

<0.01

<0.01

<0.01

0.18

Contrasts P value3

CO-P:

CR vs. CN

0.04

<0.01

<0.01

0.52

<0.01

0.02

<0.01

<0.01

0.45

LSO3:

No vs. Add

<0.01

<0.01

0.07

<0.01

<0.01

<0.01

<0.01

<0.01

0.67

Pea:

High vs. Low

<0.01

<0.01

<0.01

<0.01

<0.01

<0.01

<0.01

<0.01

<0.01

  1. a-fMeans with the different letters in the same column are significantly different (P < 0.05)
  2. 1BPP: blend pelleted product; BPP1: lignosulfonate 0% DM + carinata meal 50% DM + pea screenings 50.0% DM.; BPP2: lignosulfonate 4.8% DM + carinata meal 47.6% DM + pea screenings 47.6% DM; BPP3: lignosulfonate 0 % DM + carinata meal 75 % DM + pea screenings 25% DM; BPP4: lignosulfonate 4.8% DM + carinata meal 71.4% DM + pea screenings 23.8% DM; BPP5: lignosulfonate 0% DM + canola meal 50% DM + pea screenings 50.0% DM; BPP6: lignosulfonate 4.8% DM + canola meal 47.6% DM + pea screenings 47.6% DM; BPP7: lignosulfonate 0% DM + canola meal 75% DM + pea screenings 25% DM; BPP8: lignosulfonate 4.8% DM + canola meal 71.4% DM + pea screenings 23.8% DM
  3. 2SEM: Standard error of means
  4. 3CO-P: Co-Product. CR: Carinata meal. CN: Canola meal. LSO3: Lignosulfonate
  5. 4Baseline for protein spectral peak: ca. 1480–1730 cm–1; protein amide I region: ca. 1569–1730 cm–1; protein amide II region: ca. 1480–1569 cm–1; center range of amide I peak: ca. 1638–1649 cm–1; center range of amide II peak: ca. 1533–1540 cm–1; center range for α–helix: ca. 1647–1653cm–1; center range for β-sheet: ca. 1625–1631cm–1