Feed nutritional value of brewers’ spent grain residue resulting from protease aided protein removal

Table 1 Chemical composition (% of DM) of brewers’ spent grain (BSG) and residues after hydrolysis using proteases varying with enzyme doses

Item	Residue, enzyme dose^d				BSG	SEM	P <
Item	Low	Med	MedH	High			Enz	L	Q	Int	R:BSG
OM
Alcalase	95.8^a	95.2^a	94.3	94.2	94.4	0.23	0.01	0.01	0.14	0.02	0.03
Everlase	94.2^b	94.1^b	93.9	94.0	94.4			0.45	0.72		0.18
Flavourzyme	94.5^b	94.3^b	94.0	94.2	94.4			0.16	0.41		0.67
NDF
Alcalase	71.4	72.7	70.9	74.8^a	46.6	0.74	0.01	0.01	0.08	0.05	0.01
Everlase	71.6	72.6	69.2	75.0^a	46.6			0.05	0.01		0.01
Flavourzyme	69.7	69.0	70.1	71.4^b	46.6			0.06	0.21		0.01
ADF
Alcalase	30.8^a	31.4^a	31.1^a	32.1	20.8	0.43	0.01	0.07	0.70	0.02	0.01
Everlase	28.5^b	29.0^b	29.1^b	31.5	20.8			0.01	0.05		0.01
Flavourzyme	28.9^b	28.2^b	30.0^a	31.0	20.8			0.01	0.11		0.01
HC
Alcalase	40.6	41.2^b	39.8	42.6^a	25.8	0.71	0.01	0.12	0.10	0.12	0.01
Everlase	43.1	43.6^a	40.0	43.5^a	25.8			0.44	0.02		0.01
Flavourzyme	40.8	40.8^c	40.1	40.4^b	25.8			0.52	0.75		0.01
NFC
Alcalase	9.1^a	8.9^a	9.2^a	6.2^a	18.1	0.82	0.01	0.03	0.09	0.01	0.01
Everlase	5.9^b	6.4^b	10.1^a	6.0^a	18.1			0.30	0.01		0.01
Flavourzyme	0.5^c	0.7^c	0.2^b	1.5^b	18.1			0.43	0.47		0.01
FA
Alcalase	0.35	0.39^ab	0.39^a	0.43	0.30	0.018	0.01	0.02	0.92	0.12	0.01
Everlase	0.34	0.43^a	0.35^b	0.40	0.30			0.19	0.32		0.01
Flavourzyme	0.32	0.34^b	0.30^c	0.35	0.30			0.32	0.32		0.21
CP
Alcalase	9.3^c	7.3^c	8.0^b	6.8^b	25.6	0.49	0.01	0.01	0.41	0.01	0.01
Everlase	10.9^b	9.2^b	8.9^b	6.7^b	25.6			0.01	0.76		0.01
Flavourzyme	18.6^a	19.0^a	17.7^a	15.0^a	25.6			0.01	0.01		0.01

OM organic matter, NDF neutral detergent fibre, ADF acid detergent fibre, HC hemicellulose, NFC non-fibre carbohydrate, FA ferulic acid, CP crude protein
Enz, enzyme effects; L, Q, linear and quadratic effects of enzyme dose (enzyme:protein, w/w; 1:100, 5:100, 10:100, 15:100, respectively, for Low, Med, MedH and High); Int, interaction between enzyme and enzyme dosage; R:BSG, contrast between average of enzyme dosage and BSG
^{a, b, c}Means with different letters in the same column and within a category differed (P < 0.05)
^dProtease dosage used in protein hydrolysis (% of BSG protein)

ISSN: 2049-1891