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Table 1 Chemical composition (% of DM) of brewers’ spent grain (BSG) and residues after hydrolysis using proteases varying with enzyme doses

From: Feed nutritional value of brewers’ spent grain residue resulting from protease aided protein removal

Item Residue, enzyme dosed BSG SEM P <
Low Med MedH High    Enz L Q Int R:BSG
OM
 Alcalase 95.8a 95.2a 94.3 94.2 94.4 0.23 0.01 0.01 0.14 0.02 0.03
 Everlase 94.2b 94.1b 93.9 94.0 94.4    0.45 0.72   0.18
 Flavourzyme 94.5b 94.3b 94.0 94.2 94.4    0.16 0.41   0.67
NDF
 Alcalase 71.4 72.7 70.9 74.8a 46.6 0.74 0.01 0.01 0.08 0.05 0.01
 Everlase 71.6 72.6 69.2 75.0a 46.6    0.05 0.01   0.01
 Flavourzyme 69.7 69.0 70.1 71.4b 46.6    0.06 0.21   0.01
ADF
 Alcalase 30.8a 31.4a 31.1a 32.1 20.8 0.43 0.01 0.07 0.70 0.02 0.01
 Everlase 28.5b 29.0b 29.1b 31.5 20.8    0.01 0.05   0.01
 Flavourzyme 28.9b 28.2b 30.0a 31.0 20.8    0.01 0.11   0.01
HC
 Alcalase 40.6 41.2b 39.8 42.6a 25.8 0.71 0.01 0.12 0.10 0.12 0.01
 Everlase 43.1 43.6a 40.0 43.5a 25.8    0.44 0.02   0.01
 Flavourzyme 40.8 40.8c 40.1 40.4b 25.8    0.52 0.75   0.01
NFC
 Alcalase 9.1a 8.9a 9.2a 6.2a 18.1 0.82 0.01 0.03 0.09 0.01 0.01
 Everlase 5.9b 6.4b 10.1a 6.0a 18.1    0.30 0.01   0.01
 Flavourzyme 0.5c 0.7c 0.2b 1.5b 18.1    0.43 0.47   0.01
FA
 Alcalase 0.35 0.39ab 0.39a 0.43 0.30 0.018 0.01 0.02 0.92 0.12 0.01
 Everlase 0.34 0.43a 0.35b 0.40 0.30    0.19 0.32   0.01
 Flavourzyme 0.32 0.34b 0.30c 0.35 0.30    0.32 0.32   0.21
CP
 Alcalase 9.3c 7.3c 8.0b 6.8b 25.6 0.49 0.01 0.01 0.41 0.01 0.01
 Everlase 10.9b 9.2b 8.9b 6.7b 25.6    0.01 0.76   0.01
 Flavourzyme 18.6a 19.0a 17.7a 15.0a 25.6    0.01 0.01   0.01
  1. OM organic matter, NDF neutral detergent fibre, ADF acid detergent fibre, HC hemicellulose, NFC non-fibre carbohydrate, FA ferulic acid, CP crude protein
  2. Enz, enzyme effects; L, Q, linear and quadratic effects of enzyme dose (enzyme:protein, w/w; 1:100, 5:100, 10:100, 15:100, respectively, for Low, Med, MedH and High); Int, interaction between enzyme and enzyme dosage; R:BSG, contrast between average of enzyme dosage and BSG
  3. a, b, cMeans with different letters in the same column and within a category differed (P < 0.05)
  4. dProtease dosage used in protein hydrolysis (% of BSG protein)