Bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics

Table 7 Effect of the dietary supplementations of Bacillus amyloliquefaciens DSM25840 on the transcriptomic profile of jejunum mucosa of weaned piglet 14 days after the Escherichia coli F4ac challenge

Name of the enriched gene set	Size, No. of genes	NES	P-value	FDR, q
Gene sets enriched in BAA respect to CO^a
STRUCTURAL_CONSTITUENT_OF_RIBOSOME	56	2.69	< 0.001	< 0.001
MITOCHONDRIAL_MEMBRANE_PART	40	2.22	< 0.001	< 0.001
MITOCHONDRIAL_RESPIRATORY_CHAIN	19	2.11	< 0.001	0.01
RIBOSOME	25	2.08	< 0.001	0.01
MITOCHONDRIAL_INNER_MEMBRANE	51	2.02	< 0.001	0.02
Gene sets enriched in CO respect to BAA^a
EXONUCLEASE_ACTIVITY	15	−2.20	< 0.001	< 0.001
EXTRACELLULAR_MATRIX_PART	42	−2.20	< 0.001	< 0.001
HELICASE_ACTIVITY	43	−2.20	< 0.001	< 0.001
PROTEINACEOUS_EXTRACELLULAR_MATRIX	74	−2.10	< 0.001	< 0.001
EXTRACELLULAR_MATRIX	75	−2.10	< 0.001	< 0.001
DNA_HELICASE_ACTIVITY	23	− 2.10	< 0.001	< 0.001
MEIOTIC_CELL_CYCLE	29	− 2.00	< 0.001	< 0.001
LIGASE_ACTIVITY	82	−2.00	< 0.001	< 0.001
CELL_CYCLE_PHASE	136	−2.00	< 0.001	0.01
DNA_REPAIR	105	−2.00	< 0.001	0.01
TRANSMEMBRANE_RECEPTOR_PROTEIN_TYROSINE _KINASE _ACTIVITY	39	−2.00	< 0.001	0.01
CHROMATIN_BINDING	26	−2.00	< 0.001	0.01
CELL_CYCLE_PROCESS	154	−2.00	< 0.001	0.01
DNA_DEPENDENT_DNA_REPLICATION	43	−2.00	< 0.001	0.01
M_PHASE	95	−2.00	< 0.001	< 0.001
COLLAGEN	17	−2.00	0.002	< 0.001
BASEMENT_MEMBRANE	28	−2.00	< 0.001	0.01
TRANSMEMBRANE_RECEPTOR_PROTEIN _KINASE_ACTIVITY	47	−2.00	< 0.001	0.01
RESPONSE_TO_DNA_DAMAGE_STIMULUS	135	−1.90	< 0.001	0.01
DNA_REPLICATION	69	−1.90	< 0.001	0.01

ISSN: 2049-1891