Bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics

Table 5 Effect of dietary supplementation of Bacillus amyloliquefaciens DSM25840 and Bacillus subtilis DSM25841 on the blood serum IgA, IgG and IgM of weaned piglets challenged with Escherichia coli F4ac

Igs	Treatments^a				SEM	P-value
Igs	BAA	BAS	AB	CO	SEM	BAA vs. CO	BAS vs. CO	AB vs. CO
Day 6, mg/mL^b
IgA	0.41	0.46	0.37	0.49	0.10	0.84	0.98	0.63
IgG	19.80	13.72	13.92	13.54	2.61	0.09	0.96	0.91
IgM	3.88	2.85	2.84	3.06	0.28	0.04	0.59	0.55
Day 12, mg/mL^c
IgA	0.72	0.46	0.42	0.54	0.09	0.51	0.85
IgG	17.94	13.6	12.57	15.84	2.24	0.55	0.48	0.31
IgM	3.75	3.96	3.03	3.38	0.54	0.66	0.45	0.64
Day 21, mg/mL^d
IgA	0.72	0.96	0.65	0.68	0.08	0.92	0.04	0.98
IgG	10.57	11.58	11.88	12.14	1.35	0.45	0.71	0.89
IgM	2.86	3.25	2.75	2.82	0.32	0.93	0.34	0.88

^aBAA B. amyloliquefaciens DSM25840, BAS B. subtilis DSM25841, AB Antibiotic, CO Control
^bNumber of animals was 16 for BAA, BAS and CO groups; 15 for AB group
^cNumber of animals was: 14 for BAA; 15 for BAS, AB and CO.
^dNumber of animals was: 6 for BAA; 9 for BAS; 10 for AB; 6 for CO

ISSN: 2049-1891