Bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics

Table 4 Effect of dietary supplementation of Bacillus amyloliquefaciens DSM25840 and Bacillus subtilis DSM25841 on the blood plasma metabolites showing statistical significances in weaned piglets challenged with Escherichia coli F4ac

Metabolite, μmol/L	Treatments^a				SEM	P-value
Metabolite, μmol/L	BAA	BAS	AB	CO	SEM	BAA vs. CO	BAS vs. CO	AB vs. CO
Day 8^b
Arg	107.0	117.1	105.2	145.3	16.8	0.02	0.11	0.02
Gly	657.3	597	785.7	893.7	72.3	0.08	0.02	0.64
Lys	191.5	225.7	188.3	246.3	19.3	0.03	0.03	0.02
Orn	58.0	65.4	63.8	82.9	11.0	0.04	0.19	0.14
Pro	228.5	216.5	242.5	292.6	23.6	0.14	0.07	0.31
Ser	111.1	101.7	116.4	133.1	6.8	0.22	0.05	0.44
Thr	91.1	123.5	78.1	180.4	28.0	0.1	0.41	0.05
Tyr	51.2	57.8	53.1	69.4	5.0	0.06	0.34	0.11
Histamine	153.4	73.1	81.5	73.4	12.5	0.07	0.85	0.98
PC.aa.C30.0	1.2	1.1	1.4	1.4	0.1	0.27	0.05	0.93
Day 21^c
Gly	1058	927.8	910.1	791.2	55.8	0.04	0.39	0.5
Gln	551	482.6	502.3	454.7	25.8	0.05	0.82	0.46
Ser	154.7	138.9	143.7	113.6	9.9	0.07	0.31	0.18
SDMA	0.7	1.0	0.9	1.3	0.2	0.07	0.51	0.22

^aBAA B. amyloliquefaciens DSM25840, BAS B. subtilis DSM25841, AB Antibiotic, CO Control
^b Number of included animals was: 14 for BAA; 15 for BAS, AB and CO.
^c Number of included animals was: 6 for BAA; 9 for BAS; 10 for AB; 6 for CO

ISSN: 2049-1891