Enterococcus faecium NCIMB 10415 administration improves the intestinal health and immunity in neonatal piglets infected by enterotoxigenic Escherichia coli K88

Table 1 Primer sequences of target and reference genes

Gene	Primer sequence (5'→3')	Product, bp	GenBank accession
TLR-9	Forward: AATCCAGTCGGAGATGTTTGCT	79	AY859728
TLR-9	Reverse: GACCGCCTGGGAGATGCT	79	AY859728
TLR-2	Forward: TCGAAAAGAGCCAGAAAACCAT	58	NM213761
TLR-2	Reverse: CTTGCACCACTCGCTCTTCA	58	NM213761
TLR-4	Forward: AGAAAATATGGCAGAGGTGAAAGC	64	GQ304754
TLR-4	Reverse: CTTCGTCCTGGCTGGAGTAGA	64	GQ304754
MyD88	Forward: GTGCCGTCGGATGGTAGTG	65	NM001099923
MyD88	Reverse: TCTGGAAGTCACATTCCTTGCTT	65	NM001099923
TRAF-6	Forward: GCTGCATCTATGGCATTTGAAG	70	AJ606305.1
TRAF-6	Reverse: CCACAGATAACATTTGCCAAAGG	70	AJ606305.1
NF-κB	Forward: TGCTGGACCCAAGGACATG	60	AK348766.1
NF-κB	Reverse: CTCCCTTCTGCAACAACACGTA	60	AK348766.1
IL-6	Forward: GATGCTTCCAATCTGGGTTCA	62	M80258.1
IL-6	Reverse: CACAAGACCGGTGGTGATTCT	62	M80258.1
Claudin-1	Forward: TCTTAGTTGCCACAGCATGG	106	NM001244539
Claudin-1	Reverse: CCAGTGAAGAGAGCCTGACC	106	NM001244539
Occludin	Forward: TTCATTGCTGCATTGGTGAT	113	NM001163647
Occludin	Reverse: ACCATCACACCCAGGATAGC	113	NM001163647
ZO-1	Forward: CCGCCTCCTGAGTTTGATAG	97	AJ318101
ZO-1	Reverse: CAGCTTTAGGCACTGTGCTG	97	AJ318101
β-actin	Forward: GGCGCCCAGCACGAT	66	DQ845171.1
β-actin	Reverse: CCGATCCACACGGAGTACTTG	66	DQ845171.1

TLR Toll-like receptor, MyD88 myeloid differentiation factor 88, TRAF-6 TNF receptor-associated factor 6, NF-κB nuclear transcription factor kappa B, IL interleukin, ZO-1 zonula occludens-1

ISSN: 2049-1891