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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression

Fig. 3

Deacetylation of spindle α-tubulin in IVM oocytes after SIRT1 activation. a Representative images of immunolocalised acetylated α-tubulin (ac-tub) in BML-278-treated oocytes. Scale bar represents 25 μm. b Image analysis of ac-tub in IVM oocytes. The quantification of integrated density parameter via ImageJ software was performed. The integrated density of BML-278-treated oocytes is normalised to the mean of the signal in vehicle control oocytes (VC) and expressed as a median ± quantiles from five independent experiments (N ≥ 35 per group). c Quantification of integrated density of oocytes treated with sirtinol (1 and 10 μmol/L), a SIRT1 inhibitor. Differences between individual group pairs were assessed post-hoc using multiple comparisons of mean ranks [30], including a Bonferroni adjustment for multiple testing; *, ** and *** denote significance at P ≤ 0.05, 0.01 and 0.005, respectively

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