In vivo and in vitro protective effect of arginine against intestinal inflammatory response induced by Clostridium perfringens in broiler chickens

Table 3 Gene expression of CATs and arginine catabolic enzymes in the jejunum of broiler chickens

Items^c		CAT ^d -1	CAT-2	CAT-3	iNOS ^e	ARG2 ^f	ADC ^g	AGAT ^h
CON		1.00	1.03	1.01^a	1.02^a	1.02	1.03^a	1.01
ARG		1.05	0.64	0.98^a	1.02^a	0.79	0.93^a	0.59
CON+CP		1.38	1.01	1.28^b	1.73^b	1.31	1.35^b	1.03
ARG + CP		1.09	0.61	0.89^a	0.93^a	0.99	0.79^a	0.92
SEM		0.049	0.063	0.047	0.087	0.067	0.066	0.056
Main effect
Arg	–	1.21	1.02	1.15	1.38	1.16	1.19	1.02
Arg	+	1.07	0.62	0.93	0.98	0.88	0.87	0.76
CP	–	1.03	0.84	0.99	1.02	0.92	0.98	0.80
CP	+	1.23	0.81	1.09	1.33	1.18	1.09	0.98
P-value
Arg		0.169	0.001	0.008	0.003	0.028	0.006	0.008
CP		0.022	0.789	0.209	0.018	0.048	0.432	0.059
Interaction		0.060	0.994	0.023	0.003	0.661	0.041	0.089

^a,bDifferent letters indicate significant differences for the interaction effect (P ≤ 0.05)
^cCON group, received a basal diet; ARG group, fed a basal diet supplemented with 3 g/kg arginine; CON+CP group, received a basal diet and underwent C. perfringens challenge; ARG + CP group, given a basal diet supplemented with 3 g/kg arginine and underwent C. perfringens challenge
^dCAT Cationic amino acid transporter
^eiNOS Inducible nitric oxide synthase
^fARG2 Arginase2
^gADC Arginine decarboxylase
^hAGAT Arginine:glycine amidinotransferase

ISSN: 2049-1891