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Table 1 Sperm quality parameters in pre-frozen (P-F) and frozen–thawed (F-T) sperm, 30 (F-T-30 min) and 240 (F-T-240 min) min after thawing (mean ± SEM)

From: GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm

Parameter Classification P-F F-T-30 min F-T-240 min
% Total motile spermatozoa GFE 72.52 ± 4.49a,1 30.10 ± 1.91a,2 8.14 ± 1.29a,3
PFE 71.45 ± 6.53a,1 4.45 ± 1.47b,2 1.97 ± 0.34b,2
% Progressive motile spermatozoa GFE 54.25 ± 2.60a,1 21.04 ± 1.77a,2 5.02 ± 1.48a,3
PFE 50.09 ± 5.01a,1 1.83 ± 0.99b,2 0.28 ± 0.09b,2
% SYBR-14+ /PI spermatozoa GFE 81.20 ± 1.65a,1 28.77 ± 2.85a,2 21.56 ± 3.46a,3
PFE 79.84 ± 3.42a,1 5.60 ± 1.56b,2 3.79 ± 1.01b,2
% M540/YO-PRO-1 spermatozoa GFE 75.13 ± 3.23a,1 24.36 ± 2.33a,2 14.59 ± 2.49a,3
PFE 73.32 ± 3.58a,1 6.26 ± 1.51b,2 2.72 ± 0.72b,2
% JC1agg spermatozoa GFE 79.14 ± 3.04a,1 34.29 ± 2.36a,2 27.57 ± 5.51a,2
PFE 77.50 ± 3.28a,1 16.50 ± 3.28b,2 12.75 ± 5.88a,2
% E/YO-PRO-1 spermatozoa GFE 80.01 ± 2.23a,1 21.92 ± 2.26a,2 14.09 ± 3.15a,3
PFE 76.10 ± 2.79a,1 8.86 ± 1.10b,2 3.39 ± 1.82b,2
  1. Each ejaculate was classified as having good (GFE) or poor freezability (PFE). Different superscript numbers (1,2,3) indicate significant differences (P < 0.05) between conditions (pre-frozen (P-F), F-T-30 min and F-T-240 min). Different superscript letters (a,b) indicate significant differences(P < 0.05) between GFE and PFE in a given parameter