Fig. 5

The expression levels of Tollip and inflammatory cytokines after Tollip interference. a The mRNA and protein levels of Tollip in HD11 cells transfected with control siRNA (si-Ctrl) or siRNA against Tollip (si-Tollip). The mRNA expression of four downstream pro-inflammatory mediators including NF-κB (b), TNF-α (c), IL-6 (d), and IL-1β (e) in HD11 cells, which were transfected with si-Ctrl or si-Tollip for 24 h, and then stimulated with LPS for another 3 h. The data are expressed as the mean ± SEM from three independent experiments performed in triplicate. ^*P<0.05, ^**P<0.01, ^***P<0.001