Fig. 3

Tollip is a target of gga-miR-1306-5p. a The sequence alignment of miR-1306-5p and one target site in the 3’UTR of Tollip are shown. b The illustration demonstrates the binding between the target of Tollip 3’UTR and gga-miR-1306-5p. c HEK293 cells were cotransfected with 3 μg of psiCHECK-2 or Tollip 3’UTR luciferase reporter plasmid (WT or MT), along with control mimics or gga-miR-1306-5p mimics as indicated. Firefly luciferase activity was measured and normalized by Renilla luciferase activity to finally calculate the Relative luciferase activity, after 36 h post transfection. d Effects of gga-miR-1306-5p mimics on Tollip expression at both protein and mRNA levels compared with negative control. e Effects of gga-miR-1306-5p inhibitor on Tollip expression at both protein and mRNA levels compared with control inhibitor. ^*P<0.05, ^**P<0.01, ^***P<0.001