Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli

Table 5 The relative mRNA expression of genes in ileal mucosa of weaned pigs fed diets supplemented with Bacillus subtilis

Item^c	E. coli challenge				SEM	P-value
Item^c	Negative control	Positive control	Low dose Bacillus subtilis	High dose Bacillus subtilis	SEM	Diet	Lin.^d	Quad.^d
d 5 PI
IFNG	1.00	1.25	1.14	0.75	0.26	0.44	0.14	0.60
IL1A	1.00^b	1.98^a	1.47^ab	1.13^ab	0.28	< 0.05	0.07	0.82
IL1B	1.00^b	4.40^a	2.79^ab	3.25^a	0.82	< 0.05	0.28	0.24
IL6	1.00	0.99	1.00	1.10	0.16	0.95	0.62	0.83
IL7	1.00^b	4.83^a	2.97^ab	3.42^a	1.10	< 0.05	0.20	0.22
MUC2	1.00	1.53	2.04	1.69	0.32	0.23	0.76	0.37
PTGS2	1.00	1.07	1.12	1.00	0.05	0.29	0.35	0.19
TNFA	1.00^b	1.92^ab	1.24^ab	2.06^a	0.31	< 0.05	0.78	0.07
d 11 PI
IFNG	1.00	2.33	1.41	1.35	0.68	0.50	0.28	0.56
IL1A	1.00	1.92	1.69	1.20	0.47	0.52	0.32	0.82
IL1B	1.00^b	3.32^a	1.59^ab	3.83^a	0.99	< 0.05	0.73	0.13
IL6	1.00^b	1.89^a	0.72^b	0.64^b	0.22	< 0.05	< 0.05	0.09
IL7	1.00^b	3.56^a	2.37^ab	4.00^a	1.00	< 0.05	0.78	0.27
MUC2	1.00	1.61	2.30	2.35	0.54	0.27	0.35	0.64
PTGS2	1.00	1.19	1.12	1.14	0.06	0.26	0.64	0.61
TNFA	1.00^b	3.46^a	1.94^ab	3.42^a	1.24	< 0.05	0.97	0.12

^a,bMeans without a common superscript are different (P < 0.05); bold P values denote statistical significance at the P < 0.05
^cEach least squares mean represents 6 observations. PI post-inoculation, IFNG Interferon gamma; IL1A Interleukin-1 alpha, IL1B Interleukin 1 beta, IL6 Interleukin 6, IL7 Interleukin-7, MUC2 Mucin 2, PTGS2 Cyclooxygenase 2, TNF Tumor necrosis factor alpha
^dLinear and quadratic effects of adding Bacillus subtilis to the control diet in pigs infected with F18 E. coli

ISSN: 2049-1891