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Table 5 The relative mRNA expression of genes in ileal mucosa of weaned pigs fed diets supplemented with Bacillus subtilis

From: Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli

Itemc E. coli challenge SEM P-value
Negative control Positive control Low dose Bacillus subtilis High dose Bacillus subtilis Diet Lin.d Quad.d
d 5 PI
IFNG 1.00 1.25 1.14 0.75 0.26 0.44 0.14 0.60
IL1A 1.00b 1.98a 1.47ab 1.13ab 0.28 < 0.05 0.07 0.82
IL1B 1.00b 4.40a 2.79ab 3.25a 0.82 < 0.05 0.28 0.24
IL6 1.00 0.99 1.00 1.10 0.16 0.95 0.62 0.83
IL7 1.00b 4.83a 2.97ab 3.42a 1.10 < 0.05 0.20 0.22
MUC2 1.00 1.53 2.04 1.69 0.32 0.23 0.76 0.37
PTGS2 1.00 1.07 1.12 1.00 0.05 0.29 0.35 0.19
TNFA 1.00b 1.92ab 1.24ab 2.06a 0.31 < 0.05 0.78 0.07
d 11 PI
IFNG 1.00 2.33 1.41 1.35 0.68 0.50 0.28 0.56
IL1A 1.00 1.92 1.69 1.20 0.47 0.52 0.32 0.82
IL1B 1.00b 3.32a 1.59ab 3.83a 0.99 < 0.05 0.73 0.13
IL6 1.00b 1.89a 0.72b 0.64b 0.22 < 0.05 < 0.05 0.09
IL7 1.00b 3.56a 2.37ab 4.00a 1.00 < 0.05 0.78 0.27
MUC2 1.00 1.61 2.30 2.35 0.54 0.27 0.35 0.64
PTGS2 1.00 1.19 1.12 1.14 0.06 0.26 0.64 0.61
TNFA 1.00b 3.46a 1.94ab 3.42a 1.24 < 0.05 0.97 0.12
  1. a,bMeans without a common superscript are different (P < 0.05); bold P values denote statistical significance at the P < 0.05
  2. cEach least squares mean represents 6 observations. PI post-inoculation, IFNG Interferon gamma; IL1A Interleukin-1 alpha, IL1B Interleukin 1 beta, IL6 Interleukin 6, IL7 Interleukin-7, MUC2 Mucin 2, PTGS2 Cyclooxygenase 2, TNF Tumor necrosis factor alpha
  3. dLinear and quadratic effects of adding Bacillus subtilis to the control diet in pigs infected with F18 E. coli