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Table 4 The relative mRNA expression of genes in jejunal mucosa of weaned pigs fed diets supplemented with Bacillus subtilis

From: Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli

Itemc E. coli challenge SEM P-value
Negative control Positive control Low dose Bacillus subtilis High dose Bacillus subtilis Diet Lin.d Quad.d
d 5 PI
CFTR 1.00b 0.99b 1.36ab 2.57a 0.43 < 0.05 < 0.05 0.52
CLDN1 1.00 0.60 1.41 1.81 0.62 0.45 0.09 0.73
MUC2 1.00b 2.58a 0.87b 1.37b 0.27 0.08 < 0.05 < 0.05
OCLN 1.00 1.14 1.17 1.65 0.33 0.33 0.08 0.47
SLC5A10 1.00b 2.41a 1.69ab 1.82ab 0.37 < 0.05 0.33 0.38
ZO1 1.00b 0.78b 1.33ab 2.15a 0.45 < 0.05 < 0.05 0.77
d 11 PI
CFTR 1.00 0.60 0.81 0.87 0.22 0.65 0.41 0.80
CLDN1 1.00a 0.49b 0.65ab 0.61ab 0.15 < 0.05 0.56 0.57
MUC2 1.00a 0.39b 0.88a 0.76ab 0.21 < 0.05 < 0.05 < 0.05
OCLN 1.00 0.64 1.33 1.24 0.31 0.52 0.23 0.33
SLC5A10 1.00a 0.29b 0.62ab 0.86a 0.16 < 0.05 < 0.05 0.77
ZO1 1.00 0.68 1.10 0.78 0.24 0.62 0.78 0.24
  1. a,bMeans without a common superscript are different (P < 0.05); bold P values denote statistical significance at the P < 0.05
  2. cEach least squares mean represents 6 observations. PI post-inoculation. CFTR cystic fibrosis transmembrane conductance regulator; CLDN1 Claudin 1; MUC2 Mucin-2; OCLN Occludin; SLC5A10 soluble carrier family 5 member 10; ZO1 Zonula occludens-1
  3. dLinear and quadratic effects of adding Bacillus subtilis to the control diet in pigs infected with F18 E. coli