Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli

Table 4 The relative mRNA expression of genes in jejunal mucosa of weaned pigs fed diets supplemented with Bacillus subtilis

Item^c	E. coli challenge				SEM	P-value
Item^c	Negative control	Positive control	Low dose Bacillus subtilis	High dose Bacillus subtilis	SEM	Diet	Lin.^d	Quad.^d
d 5 PI
CFTR	1.00^b	0.99^b	1.36^ab	2.57^a	0.43	< 0.05	< 0.05	0.52
CLDN1	1.00	0.60	1.41	1.81	0.62	0.45	0.09	0.73
MUC2	1.00^b	2.58^a	0.87^b	1.37^b	0.27	0.08	< 0.05	< 0.05
OCLN	1.00	1.14	1.17	1.65	0.33	0.33	0.08	0.47
SLC5A10	1.00^b	2.41^a	1.69^ab	1.82^ab	0.37	< 0.05	0.33	0.38
ZO1	1.00^b	0.78^b	1.33^ab	2.15^a	0.45	< 0.05	< 0.05	0.77
d 11 PI
CFTR	1.00	0.60	0.81	0.87	0.22	0.65	0.41	0.80
CLDN1	1.00^a	0.49^b	0.65^ab	0.61^ab	0.15	< 0.05	0.56	0.57
MUC2	1.00^a	0.39^b	0.88^a	0.76^ab	0.21	< 0.05	< 0.05	< 0.05
OCLN	1.00	0.64	1.33	1.24	0.31	0.52	0.23	0.33
SLC5A10	1.00^a	0.29^b	0.62^ab	0.86^a	0.16	< 0.05	< 0.05	0.77
ZO1	1.00	0.68	1.10	0.78	0.24	0.62	0.78	0.24

^a,bMeans without a common superscript are different (P < 0.05); bold P values denote statistical significance at the P < 0.05
^cEach least squares mean represents 6 observations. PI post-inoculation. CFTR cystic fibrosis transmembrane conductance regulator; CLDN1 Claudin 1; MUC2 Mucin-2; OCLN Occludin; SLC5A10 soluble carrier family 5 member 10; ZO1 Zonula occludens-1
^dLinear and quadratic effects of adding Bacillus subtilis to the control diet in pigs infected with F18 E. coli

ISSN: 2049-1891