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Table 3 Intestinal morphology of weaned pigs fed diets supplemented with Bacillus subtilis

From: Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli

Itemc E. coli challenge SEM P-value
Negative control Positive control Low dose Bacillus subtilis High dose Bacillus subtilis Diet Lin.d Quad.d
d 5 PI
Duodenum
 Villi height, μm 464 433 437 428 19.73 0.57 0.86 0.80
 Crypt depth, μm 319 294 313 301 19.08 0.79 0.81 0.52
 Villi height:Crypt depth 1.46 1.49 1.40 1.44 0.058 0.74 0.56 0.37
 Villi width, μm 150 161 138 136 12.85 0.43 0.15 0.47
 Villi area, mm2 0.066 0.058 0.059 0.058 0.004 0.59 0.99 0.82
Jejunum
 Villi height, μm 446a 361b 370b 401ab 33.58 0.038 0.18 0.69
 Crypt depth, μm 259 236 230 237 23.78 0.50 0.93 0.71
 Villi height:Crypt depth 1.74 1.54 1.62 1.70 0.123 0.49 0.26 0.99
 Villi width, μm 110 109 110 108 3.41 0.98 0.88 0.74
 Villi area, mm2 0.054a 0.038b 0.039b 0.041b 0.004 0.042 0.60 0.91
Ileum
 Villi height, μm 375 372 355 359 17.71 0.56 0.43 0.45
 Crypt depth, μm 206 222 219 213 8.90 0.46 0.39 0.85
 Villi height:Crypt depth 1.82 1.68 1.61 1.68 0.054 0.082 0.95 0.36
 Villi width, μm 115 114 117 109 6.37 0.85 0.58 0.53
 Villi area, mm2 0.042 0.041 0.037 0.038 0.003 0.37 0.45 0.33
d 11 PI
Duodenum
 Villi height, μm 511a 402b 439b 436b 32.75 0.015 0.28 0.46
 Crypt depth, μm 388a 330b 314b 319b 39.50 0.012 0.62 0.58
 Villi height:Crypt depth 1.33 1.23 1.41 1.37 0.077 0.17 0.091 0.14
 Villi width, μm 159 163 142 139 9.53 0.086 0.039 0.31
 Villi area, mm2 0.077 0.073 0.064 0.079 0.019 0.84 0.74 0.43
Jejunum
 Villi height, μm 448 405 385 441 25.80 0.14 0.22 0.16
 Crypt depth, μm 269 252 248 254 14.51 0.76 0.93 0.78
 Villi height:Crypt depth 1.70 1.61 1.55 1.74 0.086 0.40 0.28 0.24
 Villi width, μm 122 111 112 115 4.40 0.31 0.55 0.77
 Villi area, mm2 0.054 0.045 0.042 0.052 < 0.01 0.077 0.17 0.16
Ileum
 Villi height, μm 405 344 391 369 28.86 0.17 0.38 0.16
 Crypt depth, μm 229 203 237 224 13.30 0.32 0.27 0.16
 Villi height:Crypt depth 1.77 1.70 1.67 1.65 0.088 0.62 0.65 0.94
 Villi width, μm 128 120 122 126 6.26 0.92 0.53 0.95
 Villi area, mm2 0.047 0.040 0.047 0.045 < 0.01 0.47 0.35 0.31
  1. a,bMeans without a common superscript are different (P < 0.05); bold P values denote statistical significance at the P < 0.05
  2. cEach least squares mean represents 6 observations. PI post-inoculation
  3. dLinear and quadratic effects of adding Bacillus subtilis to the control diet in pigs infected with F18 E. coli