Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli

Kim, Kwangwook; He, Yijie; Xiong, Xia; Ehrlich, Amy; Li, Xunde; Raybould, Helen; Atwill, Edward R.; Maga, Elizabeth A.; Jørgensen, Jens; Liu, Yanhong

doi:10.1186/s40104-019-0364-3

Journal of Animal Science and Biotechnology

Table 3 Intestinal morphology of weaned pigs fed diets supplemented with Bacillus subtilis

From: Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli

Item^c	E. coli challenge				SEM	P-value
Item^c	Negative control	Positive control	Low dose Bacillus subtilis	High dose Bacillus subtilis	SEM	Diet	Lin.^d	Quad.^d
d 5 PI
Duodenum
Villi height, μm	464	433	437	428	19.73	0.57	0.86	0.80
Crypt depth, μm	319	294	313	301	19.08	0.79	0.81	0.52
Villi height:Crypt depth	1.46	1.49	1.40	1.44	0.058	0.74	0.56	0.37
Villi width, μm	150	161	138	136	12.85	0.43	0.15	0.47
Villi area, mm²	0.066	0.058	0.059	0.058	0.004	0.59	0.99	0.82
Jejunum
Villi height, μm	446^a	361^b	370^b	401^ab	33.58	0.038	0.18	0.69
Crypt depth, μm	259	236	230	237	23.78	0.50	0.93	0.71
Villi height:Crypt depth	1.74	1.54	1.62	1.70	0.123	0.49	0.26	0.99
Villi width, μm	110	109	110	108	3.41	0.98	0.88	0.74
Villi area, mm²	0.054^a	0.038^b	0.039^b	0.041^b	0.004	0.042	0.60	0.91
Ileum
Villi height, μm	375	372	355	359	17.71	0.56	0.43	0.45
Crypt depth, μm	206	222	219	213	8.90	0.46	0.39	0.85
Villi height:Crypt depth	1.82	1.68	1.61	1.68	0.054	0.082	0.95	0.36
Villi width, μm	115	114	117	109	6.37	0.85	0.58	0.53
Villi area, mm²	0.042	0.041	0.037	0.038	0.003	0.37	0.45	0.33
d 11 PI
Duodenum
Villi height, μm	511^a	402^b	439^b	436^b	32.75	0.015	0.28	0.46
Crypt depth, μm	388^a	330^b	314^b	319^b	39.50	0.012	0.62	0.58
Villi height:Crypt depth	1.33	1.23	1.41	1.37	0.077	0.17	0.091	0.14
Villi width, μm	159	163	142	139	9.53	0.086	0.039	0.31
Villi area, mm²	0.077	0.073	0.064	0.079	0.019	0.84	0.74	0.43
Jejunum
Villi height, μm	448	405	385	441	25.80	0.14	0.22	0.16
Crypt depth, μm	269	252	248	254	14.51	0.76	0.93	0.78
Villi height:Crypt depth	1.70	1.61	1.55	1.74	0.086	0.40	0.28	0.24
Villi width, μm	122	111	112	115	4.40	0.31	0.55	0.77
Villi area, mm²	0.054	0.045	0.042	0.052	< 0.01	0.077	0.17	0.16
Ileum
Villi height, μm	405	344	391	369	28.86	0.17	0.38	0.16
Crypt depth, μm	229	203	237	224	13.30	0.32	0.27	0.16
Villi height:Crypt depth	1.77	1.70	1.67	1.65	0.088	0.62	0.65	0.94
Villi width, μm	128	120	122	126	6.26	0.92	0.53	0.95
Villi area, mm²	0.047	0.040	0.047	0.045	< 0.01	0.47	0.35	0.31

^a,bMeans without a common superscript are different (P < 0.05); bold P values denote statistical significance at the P < 0.05
^cEach least squares mean represents 6 observations. PI post-inoculation
^dLinear and quadratic effects of adding Bacillus subtilis to the control diet in pigs infected with F18 E. coli

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ISSN: 2049-1891

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