Fig. 6
From: Meclofenamic acid represses spermatogonial proliferation through modulating m6A RNA modification
Anti-FLAG-based RNA immunoprecipitation coupled to RT-qPCR. a Validation of Flag tagged FTO expression by Western blot. β-actin was used as the loading control. b Electrophoretogram of the RT-PCR products using immunoprecipitated RNA and Cdk2 primer. c RNA IP confirmation of FTO binding to RNAs identifed by RT-qPCR