Fig. 2

Effect of MA2 on cell apoptosis. a GC-1 cells were treated with 0, 40, 80 and 120 μmol/L MA2 for 48 h. TUNEL assay was used to analyze apoptosis. TUNEL positive cells were indicated by green fluorescence. Cell nucleic were stained by DAPI (blue). Bar = 50 μm. b GC-1 cells were double-stained with PI/Annexin V and analyzed by flow cytometry. Q4 represents non-apoptotic cells, Q3 represents apoptotic cells, and Q2 represents dead cells. c Apoptotic rates of all groups were showed in column diagram. Data were represented by mean ± SEM, (n = 3 independent replicates).d Effects of MA2 on the abundance of PARP, cleaved PARP and Bcl2. β-Actin was used as the loading control