Dietary supplementation with free methionine or methionine dipeptide mitigates intestinal oxidative stress induced by Eimeria spp. challenge in broiler chickens

Table 5 Carbonylated protein, CP, total antioxidant capacity, TAC, and antioxidant enzyme activities in the jejunum of broilers 144 h post-inoculation with Eimeria spp

		CP^d		TAC^e		Catalase^f		SOD^g
		Mean	SE	Mean	SE	Mean	SE	Mean	SE
UC^h	NS^j	1.05^bc	0.11	64.36^c	3.52	28.27^a	2.06	4.49^ab	0.09
	dl-Met^k	1.04^bc	0.17	81.89^a	2.15	21.47^bc	1.42	3.82^c	0.04
	dl-MMet^l	1.27^ab	0.16	71.39^bc	1.69	27.78^a	1.28	3.69^c	0.13
ECⁱ	NS	1.43^a	0.10	65.27^c	2.10	19.62^c	1.25	4.72^a	0.05
	dl-Met	0.85^c	0.15	67.45^c	3.06	24.77^ab	1.94	4.32^b	0.11
	dl-MMet	0.90^bc	0.07	78.32^ab	2.52	22.38^bc	0.49	3.59^c	0.14
P-value
Challenge × Diet		0.0200		0.0008		0.0012		0.0214

^a,b,cMeans in the same column followed by different letters differ significantly by the Tukey test, P < 0.05. Results are presented as mean ± standard error, SE. Each bird was considered an experimental unit, n = 6
^dExpressed as nmol of carbonyl groups/mg of protein
^eTAC = total antioxidant capacity, expressed as percentage, %
^fCatalase activity, expressed as the amount of hydrogen peroxide, H₂O₂ consumed per minute per milligram of protein
^gSOD = superoxide dismutase activity, expressed as SOD unit/mg of protein
^hUC = unchallenged broilers
ⁱEC = Eimeria-challenged broilers
^jNS = non-supplemented diet
^kdl-Met = diet supplemented with free methionine (dl-methionine 99%)
^ldl-MMet = diet supplemented with methionine dipeptide (dl-methionyl-dl-methionine 95%)

ISSN: 2049-1891