Dietary supplementation with free methionine or methionine dipeptide mitigates intestinal oxidative stress induced by Eimeria spp. challenge in broiler chickens

Table 2 Calculated and analyzed nutrient composition, g/kg, as is, of experimental diets fed to 10–20 days old broilers

Nutrients	NS^a	dl-Met	dl-MMet
Analyzed composition^b, g/kg
Crude protein	217	220	216
Lysine	12.87 (100)^c	12.78 (100)	12.59 (100)
Methionine	3.05 (24)	5.78 (45)	5.84 (46)
Methionine + Cystine	6.53 (51)	9.10 (71)	9.17 (73)
Threonine	8.54 (66)	8.73 (68)	8.41 (67)
Tryptophan	2.81 (22)	2.85 (22)	2.79 (22)
Valine	10.39 (81)	10.56 (83)	10.12 (80)
Isoleucine	9.56 (74)	9.75 (76)	9.27 (74)
Arginine	14.52 (113)	14.89 (117)	14.34 (114)
Calculated composition
AME^d, kcal/kg	3053	3052	3052
Calcium, g/kg	8.76	8.76	8.76
Available phosphorus, g/kg	4.50	4.50	4.50
Sodium, g/kg	2.00	2.00	2.00

^aNS Non-supplemented diet (control diet), dl-Met diet supplemented with dl-methionine 99%, dl-MMet diet supplemented with dl-methionyl-dl-methionine 95%
^bFeeds were formulated using the total amino acid contents of corn and soybean meal determined by near infrared reflectance spectroscopy. The total amino acid and other nutrient values are shown as gram per kilogram and not as a percentage of the diets. Total amino acid compositions were analytically determined by Evonik Industries (Hanau, Germany) using wet chemistry (high-performance liquid chromatography)
^cValues in parentheses indicate the amino acid-to-lysine ratios (ideal protein concept)
^dAME Apparent metabolizable energy

ISSN: 2049-1891