Dietary supplementation with free methionine or methionine dipeptide mitigates intestinal oxidative stress induced by Eimeria spp. challenge in broiler chickens

Table 1 Composition of experimental diets, %, as is, fed to 10–20 days old broilers

Ingredient, kg	NS^a	dl-Met	dl-MMet
Corn (7.8%)	54.89	54.89	54.89
Soybean meal (46%)	37.30	37.30	37.30
Soybean oil	3.80	3.80	3.80
L-Lysine HCl (78%)	0.16	0.16	0.16
L-Threonine (98.5%)	0.04	0.04	0.04
dl-Methionyl-dl-Methionine (95%, dipeptide)	–	–	0.29
dl-Methionine (99%)	–	0.28	–
Dicalcium phosphate (20%)	1.53	1.53	1.53
Limestone (38%)	1.16	1.16	1.16
Salt	0.45	0.45	0.45
Vitamin–mineral premix^b	0.40	0.40	0.40
Inert filler	0.30	0.02	0.01
Total	100.00	100.00	100.00

^aNS Non-supplemented diet (control diet), dl-Met diet supplemented with dl-methionine 99%, dl-MMet diet supplemented with dl-methionyl-dl-methionine 95%
^bThe diets supplied the following compounds (per kg): retinyl acetate, 3.44 mg; cholecalciferol, 50 μg; dl-α-tocopherol, 15 mg; thiamine, 1.63 mg; riboflavin, 4.9 mg; pyridoxine, 3.26 mg; cyanocobalamin, 12 μg; d-pantothenic acid, 9.8 mg; d-biotin, 0.1 mg; menadione, 2.4 mg; folic acid, 0.82 mg; niacinamide, 35 mg; selenium, 0.2 mg; iron, 35 mg; copper, 8 mg; manganese, 60 mg; zinc, 50 mg; iodine, 1 mg; and butylated hydroxy toluene, 80 mg

ISSN: 2049-1891