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Table 2 Sequences and accession numbers of oligonucleotide primers used for real-time PCR and the length of the PCR products

From: Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows

Gene name

Oligonucleotide sequences (5’to3’) of primers

GenBank accession number

Product length, bp

GAPDH

F: CCACGTTGTAGCCGACATC

NM001034034

201

R: CCCTGAAGAGGACCTGTGAG

β-actin

F: CACCGCAAATGCTTCTAGGC

NM_173979.3

186

R: TGTCACCTTCACCGTTCCAG

HPRT

F: GACCAGTCAACAGGCGACAT

NM_001034035.2

130

R: TGACCAAGGCAAGCAAAGTC

INSR

F: AGGAGCTGGAGGAGTCCTCGTTCA

XM005208817.2

147

R: CATTCCCCACGTCACCAAGGGCTC

GLUT4

F: TTCATTGGCGCCTACTCAGG

NM174604.1

142

R: CTAGCACCTGGGCGATTAGG

TNFα

F: CCACGTTGTAGCCGACATC

NM173966

155

R: CCCTGAAGAGGACCTGTGAG

PPARγ

F: ACTTTGGGATCAGCTCCGTG

NM181024.2

137

R: GTCAGCTCTTGGGAACGGAA

  1. GAPDH Glyceraldehydes 3-phosphate dehydrogenase, HPRT Hypoxanthine phosphoribosyl-transferase, INSR insulin receptor, GLUT4 Glucose transporter 4, TNFα Tumor necrosis factor-alpha, PPARγ Peroxisome proliferator-activated receptor gamma