Fig. 3
From: Application of new biotechnologies for improvements in swine nutrition and pork production
Recombinant DNA technology. With the action of restriction enzymes, a segment of DNA (insert) is isolated from a pig DNA and a bacterial DNA plasmid is cleaved. Catalyzed by a ligase, a DNA insert joins the open plasmid to create a recombinant DNA plasmid, which is then introduced into E. coli to produce a protein or polypeptide of interest. The plasmid and bacteria replicate rapidly to generate a large amount of the protein or polypeptide