Table 2 Primer sequences for the mRNA expression analysis of genes
Gene name | Primers (5′ to 3´) | Length, bp |
|---|---|---|
ActinB (β-actin) | F: ATGGCTACTGCTGCGTCGT R: TTGAAGGTGGTCTCGTGGAT | 161 |
Toll-like receptor 2 (TLR2) | F: ACGACGCCTTTGTGTCCTAC R: GGGACGAAGTCTCGCTTATG | 121 |
Toll-like receptor 3 (TLR3) | F: ATTGGGCAAGAACTCACAGG R: AGGCTTGGAACTGAGGTGAA | 119 |
Toll-like receptor 4 (TLR4) | F: ACTCCCTCCCTAGCCTTCAG R: GCCGTGATACGGTTGAAACT | 124 |
Toll-like receptor 7 (TLR7) | F: GTTCCATTTCCTTGCACACC R: GGGCACATGCTGAAGAGAGT | 123 |
Myeloid differentiation primary response 88 (MyD88) | F: GAGGACGTGCTGATGGAACT R: CGAGGGATGCTGCTGTCTAT | 125 |
Interleukin-1 receptor-associated kinase 1 (IRAK1) | F: GACACCGACACCTTCAGCTT R: TGCCTCCTCTTCAACCAAGT | 117 |
TNF receptor associated factor 6 (TRAF6) | F: CAGCAGTGCAATGGGATTTA R: CCGGGTTTGCCAGTATAGAA | 119 |
Toll like receptor adaptor molecule 1 (TRIF) | F: ACTTCTCACAGGCACCACCT R: GGTCCCTCTCTGATTCCACA | 119 |
TANK-binding kinase 1 (TBK1) | F: TGATCACGTTGGATTTCTGC R: GCTTGGTGCGTATGTCTGAA | 120 |
Nuclear factor kappa B subunit 1 (NFKB1) | F: GTGCTCGGTGGGAGTAAGAG R: CTCCCGTCACTGCATAGTCA | 119 |
NFKB inhibitor alpha (NFKB1A) | F: CTACACCTTGCCTGTGAGCA R: CACGTGTGGCCATTGTAGTT | 116 |
Interferon regulatory factor 3 (IRF3) | F: GACCAGCCATGGATCAAGAG R: CAGGTCGACAGTGTTCTCCA | 117 |
Interferon regulatory factor 7 (IRF7) | F: TGGCAGCAGATACTGGTGAG R: GAAGATGGTCCTCCAAGCAG | 129 |
Interferon beta (IFN-β) | F: CCATCATTGAGCACCTCCTT R: AGGTGAAGATCGGTCGTGTC | 118 |
Interferon gamma (IFN-γ) | F: GAACGGCAGCTCTGAGAAAC R: ATTTTGGCGACAGGTCATTC | 125 |
Transforming Growth factor beta 1 (TGF-β) | F: GAACTGCTGTGTTCGTCAGC R: TCCAGGCTCCAGATGTAAGG | 126 |
Tumor necrosis factor (TNF-α) | F: CCACTGACGGGCTTTACCT R: TGATGGCAGAGAGGATGTTG | 141 |
Interleukin 1 beta (IL-1β) | F: AAGCCTCTCCACCTCCTCTC R: TTGTCCCTGATACCCAAGG | 114 |
Interleukin 2 (IL-2) | F: GTGAAGTCATTGCTGCTGGA R: GCGTTAACCTTGGGCATGTA | 113 |
Interleukin 4 (IL-4) | F: CATCCTCACATCGCAGAAGA R: ACGCCTAAGCTCAATTCCAG | 118 |
Interleukin 6 (IL-6) | F: TGACTTCTGCTTTCCCTACCC R: GCCAGTGTCTCCTTGCTGTT | 193 |
Interleukin 10 (IL-10) | F: GCTGTTGCCTGGTCTTCCT R: TGTTCAGTTGGTCCTTCATTTG | 178 |