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Table 2 Sequences of tva-modified DF-1 clones

From: Sequential disruption of ALV host receptor genes reveals no sharing of receptors between ALV subgroups A, B, and J

ID

Sequence (5′→3′)

Indel

WT

TGCTACCCGCTGGAGTGGCTCTGCGACGGGCATCCCGACTGCGACGATGGACGGGACGAGTGGGGCTGCG

WT

TVA#1–2

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1 modif/

− 6 bp

TVA#1–5

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−3 bp

TVA#1–6

TGCTACCCGCTGGAGTGGCTCTGCGACGGGCATCCCGACTGCGACGATGGACGGGAcCGAGTGGGGCTGCG

+ 1 bp

TVA#1–7

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−42 bp

TVA#1–8

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−16 bp

TVA#4–2

TGCTACCCGCTGGAGTGGCTCTGCGACGGGCATCCCGACTGCGACGATGGACGGGACGAGTGGGGCTGCG

WT

TVA#4–4

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−13 bp

TVA#4–9

TGCTACCCGCTGGAGTGGCTCTGCGACGGGCATCCCGACTGCGACGATGGACGGGACGAGTGGGGCTGCG

WT

  1. Underlining indicates guide RNA recognition sites and protospacer-adjacent motif sequences
  2. Strikethroughs indicate deleted nucleotides and the lowercase letter indicates an inserted nucleotide. The italicized bold letter indicates a modified mutation