Table 1 Oligos used in the study
Oligo ID | Sequence (5′→3′) |
|---|---|
TVA#1 F | CACCGGCGACGATGGACGGGACGAG |
TVA#1 R | AAACCTCGTCCCGTCCATCGTCGCC |
TVA#4 F | CACCGCGCTGGAGTGGCTCTGCGAC |
TVA#4 R | AAACGTCGCAGAGCCACTCCAGCGC |
TVA seq F | ACACTGACAGCGAGGCGTGC |
TVA seq R | ACCTCTCCGCACGACGTTCT |
wtTVA F | AAGCTTCCGGCGGGCCCGGGGCCGGCATG |
wtTVA R | GCGGCCGCAGGCAAAAAGAGTGAGGGAATTCC |
mutaTVA(+ 1) F | CTGCGACGATGGACGGGACCGAGTGGGGCTGCGGAGCGAG |
mutaTVA(+ 1) R | CTCGCTCCGCAGCCCCACTCGGTCCCGTCCATCGTCGCAG |
mutaTVA(−3) F | CGACTGCGACGATGGACGGGAGTGGGGCTGCGGAGCGAGC |
mutaTVA(−3) R | GCTCGCTCCGCAGCCCCACTCCCGTCCATCGTCGCAGTCG |
mutaTVA(∆1–6) F | ATCCCGACTGCGACGATGGAAAGTGGGGCTGCGGAGCGAG |
mutaTVA(∆1–6) R | CTCGCTCCGCAGCCCCACTTTCCATCGTCGCAGTCGGGAT |