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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Sequential disruption of ALV host receptor genes reveals no sharing of receptors between ALV subgroups A, B, and J

Fig. 1

Schematic representation showing genome editing of Avian Leukosis Virus (ALV) host receptors. a DF-1 cells were transfected with CRISPR/Cas9 vectors containing Cas9 protein-coding sequences, tva-targeting guide RNA, and puromycin resistance genes. After puromycin selection a T7E1 assay and sequence analysis were performed. In addition, single tva-modified DF-1 cells were cultured the tva gene sequenced. Clones were then infected with ALV subgroup A produced by RCAS-A-GFP vector-transfected DF-1 cells. b Schematic representation showing sequential disruption of host receptors for ALV subgroups J, B, and A. TVA, tva targeting gRNA; TVB, tvb targeting gRNA; NHE1, chNHE1 targeting gRNA; U6, human U6 promoter; CBh, chicken beta-actin short promoter; Amp, ampicillin; Tk, thymidine kinase promoter; Neo, neomycin resistance gene; Puro, puromycin resistance gene

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