Fig. 1From: Manipulation of bicarbonate concentration in sperm capacitation media improves in vitro fertilisation output in porcine speciesEffect of HCO3− concentration on sperm protein kinase A substrates phosphorylation (pPKAs). A) pPKAs of sperm samples incubated for 60 min in capacitating medium (TALP) with different concentrations of HCO3− (0 mmol/L, 5 mmol/L, 15 mmol/L and 25 mmol/L) and in PBS as non-capacitating control (NCAP) in 4 replicates. β-actin (β-act) was used as loading control. B) Relative amount of semi-quantified signal for blots. Results are shown as mean ± SEM. Different letters (a, b) indicate statistically significant differences (P < 0.05)Back to article page