Skip to main content

Table 1 Diameter of hydrolysis zone formed by CFS of LAB isolates in SMAWD assay

From: Comparative studies of versatile extracellular proteolytic activities of lactic acid bacteria and their potential for extracellular amino acid productions as feed supplements

Isolates

pH 5*

Level**

pH 6.5

Level

pH 8

Level

P. pentosaceus B12m9

1.17 ± 0.03Aa

+++

1.00 ± 0.00ABCb

++

0.90 ± 0.00CDc

+

P. pentosaceus TB-1

1.20 ± 0.00Aa

+++

0.87 ± 0.03Eb

+

0.90 ± 0.00CDb

+

P. pentosaceus TL-3

1.17 ± 0.03Aa

+++

0.97 ± 0.03BCDb

+

0.97 ± 0.03BCb

+

P. pentosaceus TP-3

1.13 ± 0.03ABa

+++

0.90 ± 0.00DEb

+

0.93 ± 0.03BCb

+

P. pentosaceus TP-4

1.13 ± 0.03ABa

+++

0.93 ± 0.03CDEb

+

0.90 ± 0.00CDb

+

P. pentosaceus TP-8

1.13 ± 0.03ABa

+++

0.93 ± 0.03CDEb

+

0.90 ± 0.00CDb

+

P. acidilactici TB-2

1.13 ± 0.03ABa

+++

0.93 ± 0.03CDEb

+

0.83 ± 0.03DEb

+

P. acidilactici TP-6

1.10 ± 0.00ABa

+++

0.90 ± 0.00DEb

+

0.80 ± 0.00Ec

+

L. plantarum TL-1

1.13 ± 0.03ABa

+++

0.93 ± 0.03CDEb

+

0.90 ± 0.00CDb

+

L. plantarum TL-2

1.20 ± 0.00Aa

+++

1.00 ± 0.00ABCb

++

1.00 ± 0.00Bb

++

L. plantarum TP-2

1.13 ± 0.03ABa

+++

1.03 ± 0.03ABa

++

1.07 ± 0.03Aa

++

L. plantarum TP-5

1.20 ± 0.00Aa

+++

1.07 ± 0.03Ab

++

1.10 ± 0.00Ab

+++

L. plantarum RI11

1.13 ± 0.03ABa

+++

1.00 ± 0.00ABCb

++

0.90 ± 0.00CDv

+

L. plantarum RG11

1.07 ± 0.03Ba

++

0.93 ± 0.03CDEb

+

0.93 ± 0.03BCb

+

L. plantarum RG14

1.17 ± 0.03Aa

+++

0.97 ± 0.03BCDb

+

0.93 ± 0.03BCb

+

L. plantarum RS5

1.07 ± 0.03Ba

++

0.93 ± 0.03CDEb

+

0.93 ± 0.03BCb

+

L. plantarum I-UL4

1.13 ± 0.03ABa

+++

0.97 ± 0.03BCDb

+

0.93 ± 0.03BCb

+

  1. *Values are mean ± standard error of the mean (SEM), n = 3. Mean ± SEM within the same column that share a similar capital letter superscript (A-E) are not significantly different (P > 0.05) while means within the same row that bear a common small letter superscript (a-c) indicate no significant difference (P > 0.05)
  2. **The level of proteolytic activity was assigned based on the diameter of clear hydrolysis zone such that: ‘+’ indicates < 1.0 cm; ‘++’ indicates ≥1.0 cm but < 1.10 cm whereas ‘+++’ indicates ≥1.10 cm