Table 4 Sense and anti-sense primer sequences (5′→3′) used in qRT-PCR analysis
Target | GenBank accession | Amplicon size, bp | Primer sequence |
|---|---|---|---|
RPL4 | XM_005659862.3 | 110 | F: TTCAAGGCTCCCATTCGACC |
R: GCACTGGTTTGATGACCTGC | |||
RPL9 | XM_013978597.2 | 106 | F: GGGTTGACAAATGGTGGGGA |
R: TTGTAACGGAAGCCCAGTGT | |||
SDHA | XM_021076931.1 | 106 | F: TTGCGAACGGAACCATAAGGA |
R: CAGCCTTCCTGTAACACGCT | |||
YWHAZ | XM_001927228.7 | 105 | F: AAGAAGGGGATTGTGGATCAGTC |
R: AAGGGCCAGACCCAATCTGA | |||
TLR-4 | NM_001113039.2 | 112 | F: CGTGCAGGTGGTTCCTAACA |
R: CAGGTAGTTAAAGCTCAGGTCCA | |||
COX-1 | XM_001926129.6 | 116 | F: ACACGGCACACGACTACATC |
R: CTTCTTCCCTTTGGTCCCCAT | |||
COX-2 | NM_214321.1 | 90 | F: ATGGGTGTGAAAGGGAGGAAAG |
R: CTGGGGATCAGGGATGAACTT | |||
5-LOX | XM_021072736.1 | 103 | F: ATGCCAAATGCCACAGGGAT |
R: ATGAACAGGTTCTCCATCGCTT | |||
12/15-LOX [90] | NM_213931 | 97 | F: CAGGCTTGGTGTCGAGAGTT |
R: AGTGGCAGAGCTGTTCCTTG | |||
TNF-α | NM_214022.1 | 94 | F: CCCCAGAAGGAAGAGTTTCCA |
R: CGACGGGCTTATCTGAGGTTT | |||
IL-6 | NM_001252429.1 | 101 | F: GACCCTGAGGCAAAAGGGAA |
R: TCCACTCGTTCTGTGACTGC | |||
IL-10 [91] | NM_214041.1 | 101 | F: GACGTAATGCCGAAGGCAGA |
R: AGGGCAGAAATTGATGACAGCG |