Protein profiles of enzymatically isolated rumen epithelium in sheep fed a fibrous diet

Bond, J. J.; Donaldson, A. J.; Coumans, J. V. F.; Austin, K.; Ebert, D.; Wheeler, D.; Oddy, V. H.

doi:10.1186/s40104-019-0314-0

Journal of Animal Science and Biotechnology

Table 3 A summary of the 5 multiprotein complexes that function in oxidative phosphorylation identified in rumen epithelium

From: Protein profiles of enzymatically isolated rumen epithelium in sheep fed a fibrous diet

Uniprot No.	Ensembl No.	Gene name	Protein name	-10logP	Pep.	Cov., %
Complex I- NADH dehydrogenase (EC 1.6.5.3)
W5QB34	ENSOARP00000019929	NDUFS1	NADH-ubiquinone oxidoreductase 75 kDa subunit	200.39	21	13
W5PJ73	ENSOARP00000010493	NDUFS2	NADH dehydrogenase [ubiquinone] iron-sulfur protein 2	30.01	6	2
W5 PB27	ENSOARP00000007638	NDUFS3	NADH dehydrogenase [ubiquinone] iron-sulfur protein 3	140.41	32	9
W5PUX0	ENSOARP00000014252	NDUFV1	NADH dehydrogenase [ubiquinone] flavoprotein 1	91.04	14	8
W5NRY1	ENSOARP00000000921	NDUFV2	NADH-ubiquinone oxidoreductase core subunit V2	113.11	27	6
W5QAH8	ENSOARP00000019723	NDUFA2	NADH dehydrogenase [ubiquinone] 1 alpha subunit 2	85.83	31	3
W5PYA5	ENSOARP00000015439	NDUFA8	NADH dehydrogenase [ubiquinone] 1 alpha subunit 8	124.37	30	5
W5QBF5	ENSOARP00000020052	NDUFA10	NADH dehydrogenase [ubiquinone] 1 alpha subunit 10	45.28	9	2
W5QHN8	ENSOARP00000022238	NDUFB5	NADH dehydrogenase [ubiquinone] 1 beta subunit 5	86.49	14	2
W5PZE3	ENSOARP00000015829	NDUFB6	NADH dehydrogenase [ubiquinone] 1 beta subunit 6	86.31	38	3
W5Q1B0	ENSOARP00000016496	NDUFB8	NADH dehydrogenase [ubiquinone] 1 beta subunit 8	64.26	15	2
W5PGA3	ENSOARP00000009469	NDUFB9	NADH dehydrogenase [ubiquinone] 1 beta subunit 9	98.66	16	3
W5Q5Y9	ENSOARP00000018129	NDUFB10	NADH dehydrogenase [ubiquinone] 1 beta subunit 10	109.34	28	4
W5P9Q8	ENSOARP00000007167	NDUFC2	NADH dehydrogenase [ubiquinone] 1 subunit C2	88.79	20	2
W5PJU2	ENSOARP00000010713		NADP oxidoreductase domain	113.31	22	5
W5PJJ7	ENSOARP00000010618		NADP oxidoreductase domain	110.5	12	5
Complex II- Succinate dehydrogenase (EC 1.3.5.1)
W5Q216	ENSOARP00000016754	SDHA	Succinate dehydrogenase	203.03	31	18
W5PNR7	ENSOARP00000012093	SDHB	Succinate dehydrogenase	109.59	25	7
Complex III- Cytochrome c reductase (EC 1.10.2.2)
W5Q0F9	ENSOARP00000016195	UQCRC2	Cytochrome b-c1 complex subunit 2	274.48	55	30
W5Q5G6	ENSOARP00000017956	UQCRC1	Ubiquinol-cytochrome c reductase	272.33	50	33
W5PUP9	ENSOARP00000014181	UQCRQ	Ubiquinol-cytochrome c reductase	123.12	30	4
W5P642	ENSOARP00000005896	UQCRB	Ubiquinol-cytochrome c reductase	130.16	50	5
W5PZC9	ENSOARP00000015815	UQCRH	Cytochrome b-c1 complex subunit 6	98.94	57	3
W5P6B2	ENSOARP00000005966	UQCR10	Ubiquinol-cytochrome c reductase	92.15	44	3
W5P2X9	ENSOARP00000004779	UQCRFS1	Cytochrome b-c1 complex subunit Rieske	156.71	41	10
C9E8M7	ENSOARP00000005022	CYB5A	Cytochrome b5 type A	52.55	29	2
W5P066	ENSOARP00000003814	CYB5B	Cytochrome b5 type B	116.91	41	6
W5QCI3	ENSOARP00000020430	CYB5R3	Cytochrome b5 reductase 3	134.01	26	5
W5Q0A9	ENSOARP00000016145	CYC1	Cytochrome c1	180.21	36	10
P62896	ENSOARP00000022795	CYC	Cytochrome c	115.32	27	3
Complex IV- Cytochrome c oxidase (EC 1.9.3.1)
O78750	ENSOARP00000000004	COX2	Cytochrome c oxidase subunit 2	101.8	30	4
W5PPE8	ENSOARP00000012324	COX4I1	Cytochrome c oxidase subunit 4I1	172.49	41	11
W5NXT8	ENSOARP00000002985	COX5A	Cytochrome c oxidase subunit 5A	187.64	73	15
W5PW27	ENSOARP00000014660	COX5B	Cytochrome c oxidase subunit 5B	142.11	73	9
W5P4E7	ENSOARP00000005299	COX6B1	Cytochrome c oxidase subunit 6B1	169.46	72	14
W5P473	ENSOARP00000005225		Cytochrome-c oxidase activity	86.89	53	4
W5PC47	ENSOARP00000019109		Cytochrome-c oxidase activity	96.35	59	5
W5PNG6	ENSOARP00000011992		Cytochrome-c oxidase activity	82.17	28	3
W5Q6E1	ENSOARP00000018281		Cytochrome-c oxidase activity	86.89	52	4
W5PXG3	ENSOARP00000016418		Cytochrome-c oxidase activity	81.29	36	4
Complex V- ATP Synthase (EC 3.6.3.10 and EC 3.6.3.14)
W5P471	ENSOARP00000005223	ATP4A	ATPase H⁺/K⁺ transporting subunit 4A	49.23	6	4
W5QEA9	ENSOARP00000021057	ATP5F1	Hydrogen ion transmembrane transporter activity	65.12	10	3
W5Q5U7	ENSOARP00000018087	ATP5L	Hydrogen ion transmembrane transporter activity	84.63	28	3
W5NY50	ENSOARP00000003098	ATP5A1	ATP synthase H⁺ transporting subunit 5A1	181.52	29	14
W5PEP7	ENSOARP00000008911	ATP5B	ATP synthase H⁺ transporting subunit 5B	236	46	25
W5PP37	ENSOARP00000012213	ATP5H	ATP synthase H⁺ transporting subunit 5H	153.27	38	6
W5PF18	ENSOARP00000009033	ATP5I	ATP synthase H⁺ transporting subunit 5I	42.72	39	2

Uniprot mammalia (Uniprot No.) and Ensembl ovine accession numbers (Ensembl No.), gene and protein name, probability score (−10logP), number of peptides matched (Pep.) and sequence coverage (Cov. %) are shown

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ISSN: 2049-1891

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